Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 345712
View all wnvNS3 Protease Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant wnvNS3 Protease protein
Ser1423-Lys1470, Gly1506-Leu1689
Accession # ABD85079

Specificity

Detects West Nile NS3 Protease in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Viral wnvNS3 Protease Antibody

Detection of wnvNS3 Protease antibody by Western Blot.

Detection of wnvNS3 Protease by Western Blot.

Western blot shows lysates of Recombinant Viral wnvNS3 Protease. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral wnvNS3 Protease Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE)

Neutralization

Measured by its ability to neutralize Recombinant Viral wnvNS3 Protease (0.5 µg/mL, Catalog # 2907-SE) cleavage of the fluorogenic peptide substrate pERTKR-Amc (20 µM, Catalog # ES013). The Neutralization Dose (ND50) is typically 15 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile Tris-buffered saline, pH 7.3. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

References

  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Long Name

West Nile Virus NS3 Protease

Entrez Gene IDs

912267 (Viral)

Gene Symbol

POLY

UniProt

ABD85079 (Viral)

Additional wnvNS3 Protease Products

Product Documents for Viral wnvNS3 Protease Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Viral wnvNS3 Protease Antibody

For research use only

Citations for Viral wnvNS3 Protease Antibody

Customer Reviews for Viral wnvNS3 Protease Antibody

There are currently no reviews for this product. Be the first to review Viral wnvNS3 Protease Antibody and earn rewards!

Have you used Viral wnvNS3 Protease Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies