Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 345713
View all wnvNS3 Protease Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant West Nile Virus NS3 Protease, expressed as a fusion protein with co-factor NS2L
Gly1506-Leu1689
Accession # ABD85079

Specificity

Detects viral wnvNS3 Protease in direct ELISAs and Western blots. Recognizes an epitope in the NS3 domain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Viral wnvNS3 Protease Antibody

Detection of wnvNS3 Protease antibody by Western Blot.

Detection of wnvNS3 Protease by Western Blot.

Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB2907) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral wnvNS3 Protease Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the co‑factor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

References

  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Long Name

West Nile Virus NS3 Protease

Entrez Gene IDs

912267 (Viral)

Gene Symbol

POLY

UniProt

ABD85079 (Viral)

Additional wnvNS3 Protease Products

Product Documents for Viral wnvNS3 Protease Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral wnvNS3 Protease Antibody

For research use only

Citations for Viral wnvNS3 Protease Antibody

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