Viral wnvNS3 Protease Antibody

Catalog # Availability Size / Price Qty
MAB29073-100
MAB29073-SP
Viral  wnvNS3 Protease ELISA Standard Curve.
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Viral wnvNS3 Protease Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral wnvNS3 Protease in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 991926
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived  Viral wnvNS3 Protease
sequences: rwnvNS2b 1-48, rwnvNS3 1-184
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Viral wnvNS3 Protease ELISA Standard Curve. Recombinant Viral wnvNS3 Protease protein was serially diluted 2-fold and captured by Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29073) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

References
  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name
West Nile Virus NS3 Protease
Entrez Gene IDs
912267 (Viral)
Alternate Names
wnvNS3 Protease

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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