Wnt-3a Antibody (3A6) - BSA Free
Novus Biologicals | Catalog # NBP1-19050
![Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0007.jpg "Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A6
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human Wnt-3a (19-352aa) purified from E. coli (NP_149122).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Wnt-3a Antibody (3A6) - BSA Free
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with Wnt-3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1: 293T cell lysateLane 2: Wnt3a Transfected 293T cell lysate![Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-3A6-Immunohistochemistry-Paraffin-NBP1-19050-img0004.jpg "Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]")
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) - BSA Free [NBP1-19050]
Immunohistochemistry-Paraffin: Wnt-3a Antibody (3A6) [NBP1-19050] - Human breast cancer tissue were incubated with anti-human Wn-t3a for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).![Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]](https://resources.rndsystems.com/images/products/Wnt-3a-Antibody-3A6-Western-Blot-NBP1-19050-img0006.jpg "Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]")
Western Blot: Wnt-3a Antibody (3A6)BSA Free [NBP1-19050]
Western Blot: Wnt-3a Antibody (3A6) [NBP1-19050] - The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Applications for Wnt-3a Antibody (3A6) - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:50-1:100SDS-PAGE 1:100-1:2000
Western Blot
1:1000-1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26708597)
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-3a
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Alternate Names
Wnt3a
Entrez Gene IDs
89780 (Human)
Gene Symbol
WNT3A
UniProt
Additional Wnt-3a Products
Product Documents for Wnt-3a Antibody (3A6) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Wnt-3a Antibody (3A6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Wnt-3a Antibody (3A6) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
