Organoid and 3-D Cell Culture Protocols

Human Liver Organoid Culture Protocol

This protocol outlines a method for culturing normal human liver organoids based on protocols described by Meritxell Huch (1). Starting with a suspension of isolated and dissociated human liver tissue, the reagents and

Human Lung Organoid Culture Protocol

This protocol outlines a method for culturing normal human lung organoids based on protocols described by Norman Sachs and Hans Clever (1). The procedure that is described allows for the long-term

Production of R-Spondin 1-conditioned Medium using Cultrex HA

This protocol outlines a method for the production of R-Spondin 1-conditioned medium from the Cultrex® HA-R-Spondin1-Fc293T Cell Line. Some specific tips and considerations you can find in this protocol include:

Harvesting Organoids for Biochemical Analysis

This protocol outlines a method for the harvesting of organoids from culture as well as the processing recommendations for passaging and biochemical analysis using the Cultrex® Organoid Harvesting Solution. Some specific tips and considerations you can find in this protocol include:

Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract

This protocol outlines a method for preparation of embedding organoids in Cultrex® Basement Membrane Extract (BME) and further analysis of the organoids by immunofluorescence. Some specific tips and considerations you can find in this protocol include:

Culture of Normal Human Gastric Organoids

This protocol outlines a method for the culture of normal human gastric organoids using Cultrex® Organoid Qualified Basement Membrane Extracts (BME) as a scaffold. Some specific tips and considerations you can find in this protocol include:

Culture of Mouse Enteric Organoids using Cultrex Basement Membrane Extract

This protocol outlines a method for the culture of organoid progenitor cells from normal and healthy mouse gastric, small intestine, or colon tissues using Cultrex® Organoid Qualified Basement Membrane Extract as a scaffold. Some specific tips and considerations you can find in this protocol include:

Use of Cultrex Basement Membrane Extract to Improve Take and Growth of Xenografts in Mice

This protocol outlines methods and tips to improve mouse xenograft studies using Cultrex® Basement Membrane Extract (BME). Some specific tips and considerations you can find in this protocol include: