Culture of Normal Human Gastric Organoids

This protocol outlines a method for the culture of normal human gastric organoids using Cultrex® Organoid Qualified Basement Membrane Extracts (BME) as a scaffold. Some specific tips and considerations you can find in this protocol include:

  • Considerations and tips for starting gastric organoid cultures
  • How Cultrex Organoid Qualified BME performs as a scaffold to support organoid cultures
  • Maintenance of gastric organoid cultures, including tips for successful passaging
  • Cryobanking and preservation of organoids for analysis and future experiments

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Organoid and 3-D Cell Culture Protocols