Harvesting Organoids for Biochemical Analysis

This protocol outlines a method for the harvesting of organoids from culture as well as the processing recommendations for passaging and biochemical analysis using the Cultrex® Organoid Harvesting Solution. Some specific tips and considerations you can find in this protocol include:

  • Method for the non-enzymatic depolymerization of extracellular matrix proteins for passaging and analysis of organoid cultures
  • Tips and considerations to improve organoid harvest for protein expression analysis by Western blot or qPCR
  • Organoid culture and differentiation considerations for mouse small intestine organoids

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