CD44 Antibody (8E2F3) - Azide and BSA Free

Name: CD44 Antibody (8E2F3) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80650
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80650

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human, Mouse, Rabbit (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 8E2F3

Format

Azide and BSA Free

View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD44 (628-699) expressed in E. coli. [Uniprot: P16070]

Reactivity Notes

Not reactive to rabbit per customer review.

Localization

Membrane; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD44 Antibody (8E2F3) - Azide and BSA Free (NBP1-47386) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD44 Antibody (8E2F3) - Azide and BSA Free

Western Blot: CD44 Antibody (8E2F3)Azide and BSA Free [NBP2-80650]

Western Blot: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2). Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650]

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining represe

Flow Cytometry: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650]

Flow Cytometry: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left). Image from the standard format of this antibody.

Western Blot: CD44 Antibody (8E2F3)Azide and BSA Free [NBP2-80650]

Western Blot: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis using anti-CD44 mAb against HeLa (1) and HUVE-12 (2) cell lysates. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.

Flow Cytometry: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650]

Flow Cytometry: CD44 Antibody (8E2F3) - Azide and BSA Free [NBP2-80650] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

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Applications for CD44 Antibody (8E2F3) - Azide and BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

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Product Specific Notices for CD44 Antibody (8E2F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.