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IL-6 Antibody [Biotin]

R&D Systems | Catalog # BAF206

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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Primate - Papio cynocephalus (Yellow Baboon)

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

Antibody Array Development, Array Development, Bioassay, Capture Assay, Electrochemiluminescent Assay, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), Functional Assay, Immunoassay Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-6
Pro29-Met212
Accession # P05231

Specificity

Detects human and primate IL-6 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant porcine IL‑6 is observed and less than 0.01% cross-reactivity with recombinant mouse IL-6 and recombinant rat IL-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IL-6 Antibody [Biotin]

IL-6 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑6 in Human PBMCs.

IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human/Primate IL-6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF206) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human IL-6 by Functional

Detection of Human IL-6 by Functional

Differences in Functional Profiles between Three Patients with Different Clinical Outcomesgp100 209–2M spots co-spotted with anti-IFN gamma, anti-TNF alpha, anti-IL1b, and anti-IL-6 are shown for three separate patient samples. Patient 10735, who remains free of disease at this writing, displays strong IFN gamma, TNF alpha, IL-1b, and IL-6 secretion. Patients 10710 and 10737 show strong IFN gamma secretion, but weak to no TNF alpha, IL-1b, and IL-6 secretion; both patients experienced disease recurrence soon after these samples were drawn. Note the diffuse pattern of IL-1b and IL-6 capture, which differs from the focal capture of IFN gamma and TNF alpha. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/16162034), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human IL-6 ELISA Standard Curve

Human IL-6 ELISA Standard Curve

Recombinant Human IL‑6 (Catalog # 206-IL) was serially diluted and captured by Mouse Anti-Human/Primate IL‑6 Monoclonal Antibody (Catalog # MAB206) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for IL-6 Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensin

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑6 (Catalog # 206-IL)

Human/Primate IL-6 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate IL‑6 Antibody (Catalog # MAB206)
  • Standard: Recombinant Human IL-6 Protein (Catalog # 206-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using BAF206 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P05231

Additional IL-6 Products

Product Documents for IL-6 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for IL-6 Antibody [Biotin]

For research use only

Citations for IL-6 Antibody [Biotin]

Customer Reviews for IL-6 Antibody [Biotin] (2)

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  • Human/Primate IL-6 Biotinylated Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2018
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID 22813435
    Species: Human
    Verified Customer | Posted 01/12/2015

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