Q: Do your HRP-conjugated antibodies contain sodium azide?

A: No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.

Q: How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?

A: Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).

Q: I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.

A: At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

Q: What is the molecular weight of your antibodies?

A: All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).

Q: Why are many of your antibodies formulated with sodium azide and BSA?

A: Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.

AKT1 [p Ser473] Antibody (0113) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-21480

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Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0113 expressed in HEK293

Format

Azide and BSA Free

View all Akt1 Primary Antibodies »

Product Specifications

Immunogen

The antibody is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues around Ser473 of human AKT1.

Modification

p Ser473

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKT1 [p Ser473] Antibody (0113) - Azide and BSA Free

Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-

Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.

Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-

Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.

Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-

Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.

Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-

Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).

Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-

Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.

Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] -

Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.

Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] -

Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.

View 4 more images

Applications for AKT1 [p Ser473] Antibody (0113) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

1:100-1:400

Immunohistochemistry-Paraffin

1:100-1:400

Western Blot

1:1000-1:200000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Akt1

AKT (also known as protein kinase B (PKB) and RAC (related to A and C kinases)) is a critical intracellular serine/threonine kinase that translates signals from extracellular stimuli including growth factors, cytokines and neurotransmitters (1). AKT signaling plays critical roles in cell growth, proliferation, survival and differentiation (1). It is also involved in organogenesis, angiogenesis and metabolism. Three mammalian AKT isoforms have been identified. The AKT pathway can be activated by any of the three members who share a high level of protein homology but are independently encoded by AKT1 (PKB alpha; 14q32.32), AKT2 (PKB beta; 19q13.2), or AKT3 (PKB gamma; 1q44) (1, 2). Each AKT family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. AKT mediates many of the downstream events of phosphatidylinositol 3-kinase (PI3-K), a lipid kinase activated by growth factors, cytokines and insulin. PI3-K recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. AKT has two main phosphorylation sites (Ser473 and Thr308, predicted molecular weight 56 kDa) (3, 4). Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus including mammalian target of rapamycin (mTOR).

The main function of AKT is to control inhibition of apoptosis and promote cell proliferation. Survival factors can activate AKT Ser473 and Thr308 phosphorylation sites in a transcription-independent manner, resulting in the inactivation of apoptotic signaling transduction through the tumor suppressor PTEN, an antagonist to PI3-K (5). PTEN exerts enzymatic activity as a phosphatidylinositol-3,4,5-trisphosphate (PIP3) phosphatase, opposing PI3K activity by decreasing availability of PIP3 to proliferating cells, leading to overexpression and inappropriate activation of AKT noted in many types of cancer.

AKT1 function has been linked to overall physiological growth and function (2). AKT1 has been correlated with proteus syndrome, a rare disorder characterized by overgrowth of various tissues caused by a mosaic variant in the AKT1 gene in humans.

AKT2 is strongly correlated with Type II diabetes, including phenotypes of insulin resistance, hyperglycemia and atherosclerosis (2, 6).

The function of AKT3 is specifically associated to brain development, where disruptions to AKT3 are correlated with microcephaly, hemimegalencephaly, megalencephaly and intellectual disabilities (2).

References

1. Ersahin, T., Tuncbag, N., & Cetin-Atalay, R. (2015). The PI3K/AKT/mTOR interactive pathway. Mol Biosyst, 11(7), 1946-1954. doi:10.1039/c5mb00101c

2. Cohen, M. M., Jr. (2013). The AKT genes and their roles in various disorders. Am J Med Genet A, 161a(12), 2931-2937. doi:10.1002/ajmg.a.36101

3. Georgescu, M. M. (2010). PTEN Tumor Suppressor Network in PI3K-Akt Pathway Control. Genes Cancer, 1(12), 1170-1177. doi:10.1177/1947601911407325

4. Mishra, P., Paital, B., Jena, S., Swain, S. S., Kumar, S., Yadav, M. K.,... Samanta, L. (2019). Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NFkB/AKT/mTOR/KEAP1 signalling in rat heart. Sci Rep, 9(1), 7408. doi:10.1038/s41598-019-43320-5

5. Wedel, S., Hudak, L., Seibel, J. M., Juengel, E., Oppermann, E., Haferkamp, A., & Blaheta, R. A. (2011). Critical analysis of simultaneous blockage of histone deacetylase and multiple receptor tyrosine kinase in the treatment of prostate cancer. Prostate, 71(7), 722-735. doi:10.1002/pros.21288

6. Rotllan, N., Chamorro-Jorganes, A., Araldi, E., Wanschel, A. C., Aryal, B., Aranda, J. F.,... Fernandez-Hernando, C. (2015). Hematopoietic Akt2 deficiency attenuates the progression of atherosclerosis. Faseb j, 29(2), 597-610. doi:10.1096/fj.14-262097

Long Name

v-Akt Murine Thymoma Viral Oncogene Homolog 1

Alternate Names

PKB alpha, PRKBA, RAC-alpha

Gene Symbol

AKT1

Additional Akt1 Products

Product Documents for AKT1 [p Ser473] Antibody (0113) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for AKT1 [p Ser473] Antibody (0113) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Intracellular Kinases

Diabetic Peripheral Neuropathy

GDNF Family

Interferons

Intracellular Kinases

Notch Pathway

PDGF Family

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for AKT1 [p Ser473] Antibody (0113) - Azide and BSA Free

Showing 1 - 5 of 5 FAQs Showing All

Q: Do your HRP-conjugated antibodies contain sodium azide?

A: No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
Q: How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?

A: Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
Q: I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.

A: At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
Q: What is the molecular weight of your antibodies?

A: All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).
Q: Why are many of your antibodies formulated with sodium azide and BSA?

A: Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.
Q: Do your HRP-conjugated antibodies contain sodium azide?

A: No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
Q: How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?

A: Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
Q: I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.

A: At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
Q: What is the molecular weight of your antibodies?

A: All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).
Q: Why are many of your antibodies formulated with sodium azide and BSA?

A: Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.
Q: Do your HRP-conjugated antibodies contain sodium azide?

A: No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
Q: How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?

A: Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
Q: I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.

A: At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
Q: What is the molecular weight of your antibodies?

A: All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).
Q: Why are many of your antibodies formulated with sodium azide and BSA?

A: Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.
Q: Do your HRP-conjugated antibodies contain sodium azide?

A: No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
Q: How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?

A: Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
Q: I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.

A: At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
Q: What is the molecular weight of your antibodies?

A: All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).
Q: Why are many of your antibodies formulated with sodium azide and BSA?

A: Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.
Q: Do your HRP-conjugated antibodies contain sodium azide?

A: No. None of our HRP-conjugated antibodies contain sodium azide as this agent inhibits the activity of HRP.
Q: How do I choose secondary antibodies to label the same cells when I have two primary antibodies from the same host?

A: Use isotype-specific secondary antibodies if the primary antibodies are of different isotypes. You can also make direct conjugates of the primary antibodies by use of antibody labeling kits, dyes, or custom conjugations (please contact Technical Support for custom orders).
Q: I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.

A: At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.
Q: What is the molecular weight of your antibodies?

A: All IgG antibodies are approximately 150 kDa (each heavy chain is about 50 kDa and each light chain is about 25 kDa).
Q: Why are many of your antibodies formulated with sodium azide and BSA?

A: Sodium azide is a preservative which is added to prevent bacterial growth. BSA is added as a protein stabilizer.