The anti-Aducanumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Aducanumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.
Anti-Aducanumab (Anti-Idiotype) Antibody (975942)
R&D Systems | Catalog # MAB9545
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Multi-Species
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 975942
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Product Specifications
Immunogen
Full length Aducanumab
Specificity
Detects Aducanumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Adalimumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, and Teprotumumab is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Anti-Aducanumab (Anti-Idiotype) Antibody (975942)
Detection of Aducanumab by Western Blot.
Western blot shows Trastuzumab (negative control), Gemtuzumab (negative control), Teprotumumab (negative control), and Aducanumab biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Aducanumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9545) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Aducanumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Aducanumab (Anti-Idiotype) Antibody in Direct ELISA.
A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Aducanumab (Anti-Idiotype) Antibody (Catalog # MAB9545, 333 ng/mL) detected Aducanumab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Adalimumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, and Teprotumumab was observed.Applications for Anti-Aducanumab (Anti-Idiotype) Antibody (975942)
Application
Recommended Usage
ELISA
Detects Aducanumab in direct ELISAs.
Western Blot
2 µg/mL
Sample: Aducanumab under non-reducing conditions only
Sample: Aducanumab under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Aducanumab
Additional Aducanumab Products
Product Documents for Anti-Aducanumab (Anti-Idiotype) Antibody (975942)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Anti-Aducanumab (Anti-Idiotype) Antibody (975942)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
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- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Anti-Aducanumab (Anti-Idiotype) Antibody (975942)
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Q: What is an anti-idiotype antibody?
A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
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