Autophagy Antibody Pack

Novus Biologicals | Catalog # NB910-94159

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Rat, Porcine, Alligator, Bovine, Canine, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Mycoplasma, Simple Western, Western Blot

Product Summary for Autophagy Antibody Pack

This pack contains 1 vial of each: NB100-2220 (0.025 mL), NB110-87318 (0.025 mL), NB110-53818 (0.025 mL), NB110-56893 (0.025 mL) and NB110-60928 (0.025 mL).

Product Specifications

Specificity

Autophagy Antibodies (Beclin, LC3, ATG5, ATG9, ATG16L1)

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Autophagy Antibody Pack

Knockout Validated: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.
Knockout Validated: Autophagy Antibody Pack [NB910-94159]

Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159]

Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Knockout Validated: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG5 was detected using NB110-53818.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94159]

Immunohistochemistry: Autophagy Antibody Pack [NB910-94159]

Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.
Simple Western: Autophagy Antibody Pack [NB910-94159]

Simple Western: Autophagy Antibody Pack [NB910-94159]

Simple Western: Autophagy Antibody Pack [NB910-94159] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Flow Cytometry: Autophagy Antibody Pack [NB910-94159]

Flow Cytometry: Autophagy Antibody Pack [NB910-94159]

Flow Cytometry: Autophagy Antibody Pack [NB910-94159] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose
Immunohistochemistry: Autophagy Antibody Pack [NB910-94159]

Immunohistochemistry: Autophagy Antibody Pack [NB910-94159]

Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.
Immunohistochemistry: Autophagy Antibody Pack [NB910-94159]

Immunohistochemistry: Autophagy Antibody Pack [NB910-94159]

Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0021.jpg
Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - Analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center.
Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.
Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG16L1. Lane 1: NIH/3T3 Lane 2: HeLa.
Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893
Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159]

Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.

Formulation, Preparation, and Storage

Formulation

See individual datasheets

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Autophagy

This Autophagy Antibodies Pack includes sample aliquots to many different autophagy related proteins.
Autophagy can be understood as a cell recycling system on its most basic level. Components of the cytoplasm are sequestered and moved in the lysosome where they are broken down into their basic components and returned to the cytosol for reuse.
For information on the individual antibodies contained in this pack, please click on their links below.

Alternate Names

ATG6, ATG6 autophagy related 6 homolog, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein), beclin 1, autophagy related, beclin1, beclin-1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197, VPS30

Gene Symbol

BECN1

Additional Autophagy Products

Product Documents for Autophagy Antibody Pack

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Autophagy Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Customer Reviews for Autophagy Antibody Pack (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse
    Species: Mouse
    Verified Customer | Posted 11/16/2011

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Protocols

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