B-Raf Antibody (CL4004) - BSA Free

Name: B-Raf Antibody (CL4004) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-59035
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-59035

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # CL4004

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P15056, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for B-Raf Antibody (CL4004) - BSA Free

Western Blot: B-Raf Antibody (CL4004) [NBP2-59035]

Western Blot: B-Raf Antibody (CL4004) [NBP2-59035] - Analysis in human cell line MOLT-4.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human testis shows cytoplasmic positivity in seminiferous tubules.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human prostate cancer shows moderate cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.

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Applications for B-Raf Antibody (CL4004) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

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Product Specific Notices for B-Raf Antibody (CL4004) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.