B-Raf Antibody (CL4004) - Azide and BSA Free

Name: B-Raf Antibody (CL4004) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-44118
Price: 539 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-44118

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # CL4004

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P15056, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for B-Raf Antibody (CL4004) - Azide and BSA Free

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human prostate cancer shows moderate cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human testis shows cytoplasmic positivity in seminiferous tubules.

Western Blot: B-Raf Antibody [NBP3-44118]

Analysis in human cell line MOLT-4.

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Applications for B-Raf Antibody (CL4004) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (CL4004) - Azide and BSA Free

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Product Specific Notices for B-Raf Antibody (CL4004) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.