B-Raf Antibody (OTI5A9)

Name: B-Raf Antibody (OTI5A9)
Brand: Novus Biologicals
SKU: NBP1-47668
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-47668

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI5A9

View all B-Raf Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

84.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf Antibody (OTI5A9)

Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668]

Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - Analysis of extracts (35ug) from 9 different cell lines by using anti-B Raf monoclonal antibody.

Immunocytochemistry/ Immunofluorescence: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunocytochemistry/Immunofluorescence: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.

Flow Cytometry: B-Raf Antibody (OTI5A9) [NBP1-47668]

Flow Cytometry: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-B Raf antibody, and then analyzed by flow cytometry.

Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668]

Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antibody

Immunohistochemistry: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunohistochemistry: B-Raf Antibody (OTI5A9) [NBP1-47668] - The images depict immunostaining of representative PTC cases harboring BRAF wild-type (A, B) and BRAF V600E mutation (C, D) proven by NGS; varying degrees of immunoreactivity for BRAF is shown both in the intranuclear inclusions (arrows) and in the cytoplasm. Original magnifications: 1,000 X.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human endometrium tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human lung tissue using anti-B Raf mouse monoclonal antibody.

Immunoprecipitation: B-Raf Antibody (OTI5A9) [NBP1-47668]

Immunoprecipitation: B-Raf Antibody (OTI5A9) [NBP1-47668] - (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Applications for B-Raf Antibody (OTI5A9)

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B-Raf

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases.This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation,and secretion. Mutations in this ge

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Entrez Gene IDs

673 (Human)

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (OTI5A9)

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Certificate of Analysis

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Product Specific Notices for B-Raf Antibody (OTI5A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.