BMP-4 Antibody (3C11C7) - BSA Free

Novus Biologicals | Catalog # NBP2-52424

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C11C7

Format

BSA Free
View all BMP-4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BMP-4 (AA: 277-408) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMP-4 Antibody (3C11C7) - BSA Free

Western Blot: BMP-4 Antibody (3C11C7)BSA Free [NBP2-52424]

Western Blot: BMP-4 Antibody (3C11C7)BSA Free [NBP2-52424]

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

Flow Cytometry: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

Flow Cytometry: BMP-4 Antibody (3C11C7) [NBP2-52424] - Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).
Western Blot: BMP-4 Antibody (3C11C7)BSA Free [NBP2-52424]

Western Blot: BMP-4 Antibody (3C11C7)BSA Free [NBP2-52424]

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: BMP-4 Antibody (3C11C7)BSA Free [NBP2-52424]

Western Blot: BMP-4 Antibody (3C11C7)BSA Free [NBP2-52424]

Western Blot: BMP-4 Antibody (3C11C7) [NBP2-52424] - Analysis using BMP4 mAb against human BMP4 recombinant protein. (Expected MW is 41 kDa)
Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

Immunocytochemistry/ Immunofluorescence: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

Immunocytochemistry/Immunofluorescence: BMP-4 Antibody (3C11C7) [NBP2-52424] - Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

ELISA: BMP-4 Antibody (3C11C7) - BSA Free [NBP2-52424]

ELISA: BMP-4 Antibody (3C11C7) [NBP2-52424] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for BMP-4 Antibody (3C11C7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready. IHC reported in scientific literature (PMID: 29306659).

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMP-4

Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

Additional BMP-4 Products

Product Documents for BMP-4 Antibody (3C11C7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BMP-4 Antibody (3C11C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BMP-4 Antibody (3C11C7) - BSA Free

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Protocols

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FAQs

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