C. tetani TeNT Light Chain Antibody

(1 citations)   
  • Species Reactivity
    C. tetani
  • Specificity
    Detects TeNT Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 604023
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant C. tetani TeNT Light Chain
    Pro2-Gly430
    Accession # P04958
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of C. tetani TeNT Light Chain by Western Blot. Western blot shows recombinant C. tetani TeNT Light Chain. PVDF Membrane was probed with 2 µg/mL of C. tetani TeNT Light Chain Monoclonal Antibody (Catalog # MAB6535) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TeNT Light Chain at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TeNT Light Chain

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length.  The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long.  The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation.  Tetanus toxin acts by inhibiting neurotransmitter release.  It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons.  It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the ’76-Gln-|-Phe-77’ bond of synaptobrevin-2. 

  • Long Name:
    Tetanus Neurotoxin Type Light Chain
  • Entrez Gene IDs:
    1061100 (C. tetani)
  • Alternate Names:
    TeNT Light Chain; TeNT-LC; Tentoxylysin; Tetanus toxin chain H; Tetanus toxin chain L; Tetanus toxin heavy chain; Tetanus toxin light chain; tetX
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Comparative pathogenomics of Clostridium tetani
    Authors: JE Cohen, R Wang, RF Shen, WW Wu, JE Keller
    PLoS ONE, 2017;12(8):e0182909.
    Species: Clostridium tetani
    Sample Type: Cell Lysates
    Application: WB
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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