cAMP Antibody

(2 citations)   
  • Specificity
    Detects cAMP. In a competition binding assay, this antibody does not cross-react with ATP, CTP, GTP, AMP, GMP, cGMP, or cUMP.
  • Source
    Monoclonal Mouse IgG1 Clone # 250532
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Thryoglobulin-coupled cAMP
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
cAMP in Human PBMCs. cAMP was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-cAMP Monoclonal Antibody (Catalog # MAB2146) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cAMP
cAMP is a cyclic nucleotide generated from ATP by the activity of adenylate cyclase. It is a ubiquitous cytoplasmic second messenger important in many signal transduction pathways.
  • Long Name:
    Cyclic AMP
  • Alternate Names:
    cAMP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway
    Authors: Gao Feng
    Mol Med Rep, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Amyloid beta (Abeta) peptide directly activates amylin-3 receptor subtype by triggering multiple intracellular signaling pathways.
    Authors: Fu W, Ruangkittisakul A, MacTavish D, Shi JY, Ballanyi K, Jhamandas JH
    J. Biol. Chem., 2012;287(22):18820-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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