CD14 Antibody (4B4F12) - BSA Free

Novus Biologicals | Catalog # NBP2-37291

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B4F12

Format

BSA Free
View all CD14 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD14 Antibody (4B4F12) - BSA Free

Dual RNAscope ISH-IHC: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Tissue sections of human tonsil were probed for CD14 mRNA (ACD RNAScope probe, catalog # 418801; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-37291) at 1:100 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
Immunocytochemistry/ Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291]

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Confocal image of hCD14-transfected HEKT cells, 4% formalin fixed, indirectly labeled with anti-CD14 1:200, and AF568-conjugated Ab 1:1000. Note that cells have different immunoreactivity due to various expression level of CD14 in single cells. Image from verified customer review.
Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291]

Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291]

Flow Cytometry: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - An intracellular stain was performed on Jurkat cells with NBP2-37291AF647 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)
Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.
Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

CD14-Antibody-4B4F12-Western-Blot-NBP2-37291-img0010.jpg
Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12)BSA Free [NBP2-37291]

Western Blot: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291]

ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291]

ELISA: CD14 Antibody (4B4F12) - BSA Free [NBP2-37291] - CD14 Antibody (4B4F12) [NBP2-37291] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CD14 Antibody (4B4F12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready. CD14 antibody validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-37291 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD14

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Alternate Names

CD14

Gene Symbol

CD14

Additional CD14 Products

Product Documents for CD14 Antibody (4B4F12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD14 Antibody (4B4F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD14 Antibody (4B4F12) - BSA Free

Customer Reviews for CD14 Antibody (4B4F12) - BSA Free (1)

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Customer Images

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  • CD14 Antibody (4B4F12)
    Name: Gabriel Wang
    Application: Immunocytochemistry
    Sample Tested: Hek 293T
    Species: Human
    Verified Customer | Posted 04/18/2018
    Confocal image of hCD14-transfected HEKT cells,4% formalin fixed, indirectly labeled with anti-CD14 1:200, and AF568-conjugated Ab 1:1000. Note that cells have different immunoreactivity due to various expression level of CD14 in single cells.
    CD14 Antibody (4B4F12) - BSA Free NBP2-37291

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Protocols

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