CD34 Antibody (MEC 14.7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80642

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Human (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # MEC 14.7

Format

Azide and BSA Free
View all CD34 Primary Antibodies »

Product Specifications

Immunogen

Murine transformed endothelioma cell line t-end.

Reactivity Notes

This antibody does not detect Human CD34 and is an excellent tool for marking host derived endothelial cells/vasculature in human cancer xenografts on mouse.

Localization

Membrane; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

Novus Biologicals Rat CD34 Antibody (MEC 14.7) - Azide and BSA Free (NB600-1071) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD34 Antibody (MEC 14.7) - Azide and BSA Free

Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which is an expected outcome for this clone. This section was included in NB600-1071's IHC validation testing as
Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor vasculature which are originating from the host (mouse). Image from the standard format of this antibody.
Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunocytochemistry/ Immunofluorescence: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - CD34 antibody was tested in WEHI-3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The staining was detected using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxy
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a FFPE tissue section of human renal cancer xenograft using CD34 antibody clone MEC 14.7 at 1:100 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent followed by counterstaining of nuclei with hematoxylin
Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a FFPE tissue section of mouse colon using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. Th
Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a FFPE tissue section of mouse heart using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. Th
Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]

Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a FFPE tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hemat

Applications for CD34 Antibody (MEC 14.7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100 - 1:250

Immunohistochemistry-Frozen

1:100 - 1:250

Immunohistochemistry-Paraffin

1:100 - 1:250

Immunoprecipitation

1:10-1:500

Western Blot

1:250
Application Notes
This CD34 (MEC 14.7) antibody is useful for Immunohistochemistry (on both paraffin-embedded and frozen sections), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation and ELISA. Antigen retrieval is required for IHC-Paraffin.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD34

CD34, also known as Cluster of Differentiation 34, is a transmembrane phosphoglycoprotein that is primarily known for being a surface marker for hematopoietic stem cells (HSCs) and progenitor cells (1,2). The CD34 protein consists of a heavily glycosylated extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with PDZ binding motifs (1,3). Human CD34 has two protein isoforms consisting of 385 amino acids (aa) for the canonical isoform and 328 aa for isoform 2 (3,4). The canonical CD34 isoform has calculated theoretical molecular weight of 40 kDa and an observed molecular weight of ~115 kDa (1,3,4). L-Selectin (CD62L) is the most common ligand for CD34, but it has also been shown to bind CrkL (1,3).

CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).

References

1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13

3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571

4. Uniprot (P28906)

5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02

6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027

Alternate Names

CD34, HPCA1

Gene Symbol

CD34

Additional CD34 Products

Product Documents for CD34 Antibody (MEC 14.7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD34 Antibody (MEC 14.7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for CD34 Antibody (MEC 14.7) - Azide and BSA Free

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    • Q: I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
      A: We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
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