Detects cotton rat CCL3/MIP‑1 alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MIP-1 alpha is observed, 15% cross-reactivity with recombinant human (rh) MIP-1 alpha is observed, less than 10% cross-reactivity with rhMIP‑1 beta, rh6Ckine, and rhHCC-4 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant cotton rat CCL3/MIP-1 alpha Ala24-Ala92 Accession # AAL26704
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061-RM)
Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 10 ng/mL Recombinant Cotton Rat CCL3/MIP‑1 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Cotton Rat CCL3/MIP‑1 alpha Antibody.
Recombinant Cotton Rat CCL3/MIP‑1 alpha (Catalog # 1061‑RM) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
MIP-1 alpha is a beta family (CC) chemokine and has been designated CCL3. MIP-1 alpha and MIP-1 beta, two closely related but distinct proteins, were originally purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Cotton rat MIP-1 alpha cDNA encodes a 92 amino acid (aa) residue precursor protein with a 23 aa putative signal peptide. Mature cotton rat MIP-1 alpha shares approximately 70% amino acid identity with human MIP-1 alpha. MIP-1 alpha is expressed in a wide variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as monocytes/macrophages.
MIP-1 alpha has been shown to play an important role in the recruitment of mononuclear cells. Additionally, MIP-1 alpha has been reported to have chemoattractant and adhesive effects on lymphocytes, preferentially promoting the chemotaxis of Th1 cells. MIP-1 alpha has also been shown to attract B cells, eosinophils, and dendritic cells. In addition, MIP-1 alpha augments cytolytic activity of NK cells (1). MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. It has been demonstrated that MIP-1 alpha can bind the chemokine receptors CCR1 and CCR5 (2).
Have you used Cotton Rat CCL3/MIP-1 alpha Antibody?
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