Digoxigenin Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB7520R-SP
MAB7520R-500
MAB7520R-01M
MAB7520R-100
Digoxigenin-Conjugated Antibody  in Human Prostate Cancer Tissue.
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Digoxigenin Antibody Summary

Specificity
Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
Source
Recombinant Monoclonal Mouse IgG2A Clone # 611621R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
KLH-coupled Digoxigenin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Digoxigenin-Conjugated Antibody  in Human Prostate Cancer Tissue. View Larger

Digoxigenin-Conjugated Antibody in Human Prostate Cancer Tissue. PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin at 10 μg/mL overnight at 4 °C, followed by Mouse Anti-Digoxigenin Recombinant Monoclonal Antibody (Catalog # MAB7520R) at 5 µg/mL for 1 hour at room temperature.Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Digoxigenin

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

References
  1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
  2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
  3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
Alternate Names
DIG; Digoxigenin

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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