Digoxigenin-Conjugated Antibody in Human Prostate Cancer Tissue. PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) conjugated to Digoxigenin at 10 μg/mL overnight at 4 °C, followed by Mouse Anti-Digoxigenin Recombinant Monoclonal Antibody (Catalog # MAB7520R) at 5 µg/mL for 1 hour at room temperature.Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.
Décarie, A.A. et al. (1994) Peptides 15(3):511.
Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.
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