Rat ERK1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00463

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
View all ERK1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat ERK1 in samples. No significant cross-reactivity or interference between Rat ERK1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.95%

Inter-Assay Precision (Precision between assays) 4.72%

Recovery for Rat ERK1 ELISA Kit (Colorimetric)

Recovery

85 - 104%

Linearity

Rat ERK1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat ERK1 ELISA Kit (Colorimetric)

Rat ERK1 ELISA Kit (Colorimetric)

ELISA: Rat ERK1 ELISA Kit (Colorimetric) [NBP3-00463] -

ELISA: Rat ERK1 ELISA Kit (Colorimetric) [NBP3-00463] - Standard Curve Reference

Kit Contents for Rat ERK1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ERK1

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Gene Symbol

MAPK3

Additional ERK1 Products

Product Documents for Rat ERK1 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat ERK1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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