ERK2 Antibody (5-D2-R)

Name: ERK2 Antibody (5-D2-R)
Brand: Novus Biologicals
SKU: NBP3-32317
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-32317

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG₁ Clone # 5-D2-R expressed in HEK293

View all ERK2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human ERK2 aa aa 200-359. (Uniprot: P28482)

Localization

Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK2 Antibody (5-D2-R)

Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317]

Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32317) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: Jurkat cell lysate (20 ug/Lane)
Lane 3: A549 cell lysate (20 ug/Lane)
Lane 4: A431 cell lysate (20 ug/Lane)
Lane 5: HepG2 cell lysate (20 ug/Lane)
Lane 6: HEK-293 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: RAW264.7 cell lysate (20 ug/Lane)
Lane 9: C6 cell lysate (20 ug/Lane)
Lane 10: PC-12 cell lysate (20 ug/Lane)

Predicted band size: 41 kDa
Observed band size: 41 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Applications for ERK2 Antibody (5-D2-R)

Application

Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK2

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for ERK2 Antibody (5-D2-R)

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Certificate of Analysis

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Product Specific Notices for ERK2 Antibody (5-D2-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.