Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Microarray

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLP-1R Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal extracellular portion of the human GLP1R protein (between residues 100-200). [UniProt P43220]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26398375).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLP-1R Antibody - BSA Free

Simple Western: GLP-1R AntibodyBSA Free [NLS1205]

Simple Western: GLP-1R AntibodyBSA Free [NLS1205]

GLP-1R-Antibody-Simple-Western-NLS1205-img0015.jpg
Immunocytochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunocytochemistry: GLP-1R Antibody - BSA Free [NLS1205]

GLP-1R-Antibody-Immunocytochemistry-NLS1205-img0014.jpg
Western Blot: GLP-1R AntibodyBSA Free [NLS1205]

Western Blot: GLP-1R AntibodyBSA Free [NLS1205]

Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.
Immunohistochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunohistochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.
Immunohistochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunohistochemistry: GLP-1R Antibody - BSA Free [NLS1205]

Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).
Simple Western: GLP-1R AntibodyBSA Free [NLS1205]

Simple Western: GLP-1R AntibodyBSA Free [NLS1205]

Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Microarray: GLP-1R Antibody - BSA Free [NLS1205]

Microarray: GLP-1R Antibody - BSA Free [NLS1205]

Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.
GLP-1R Antibody - BSA Free

Simple Western: GLP-1R Antibody - BSA Free [NLS1205] -

Simple Western: GLP-1R Antibody - BSA Free [NLS1205] - Effect of the administration of exendin‐4 on eNOS expression. (a) Capillary western blot of total eNOS & phosphorylated eNOS expression in the rat retina. The red box indicates the target protein. (b & c) Fold change in the expression of total eNOS (b) & phosphorylated eNOS (c) in the rat retina (n = 7 in control group; n = 6 in Hp + PBS group; n = 9 in other groups). (d & e) Capillary western blot & fold change in the expression of GLP‐1 receptors (GLP‐1R) (d), PI3K, & Akt (e) in the rat retina. *P <.05, significantly different from CSF group; #P <.05significantly different from noradrenaline without exendin‐4 group. (f) NO content in human retinal microvascular endothelial cells (n = 6, 5, 5, 6, 7, 5, & 6 for group of control, OGD, OGD + Ex‐4, OGD + Ex‐4 + Ex‐9–39‐L, OGD + Ex‐4 + Ex‐9–39‐H, OGD + Ex‐4 + l‐NAME‐L, & OGD + Ex‐4 + l‐NAME‐H, respectively). One‐way ANOVA with LSD or Dunnett's T3 test were performed. C, control group; HP, high pressure injury group; EX‐4, exendin‐4; s.c., subcutaneous injection of exendin‐4; i.v., intravitreal injection of exendin‐4; od, eye drops of exendin‐4; OGD, oxygen glucose deprivation model; EX‐9–39‐L, low concentration of exendin‐9‐39 (10 nM); EX‐9–39‐H, high concentration of exendin‐9‐39 (20 nM); l‐NAME‐l, low concentration of l‐NAME (50 μM); l‐NAME‐H, high concentration of l‐NAME (100 μM) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32232832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLP-1R Antibody - BSA Free

Western Blot: GLP-1R Antibody - BSA Free [NLS1205] -

PAX6 down‐regulation reduces beta cell survival under diabetic conditionAEndoC‐ beta H1 cells with PAX6 overexpression were subjected to cell proliferation measurement indexed by BrdU labeling after 72‐h exposure to normal or HGPA condition with insulin (100 nM), Exendin‐4 (10 nM), GIP (10 nM), or IGF1 (50 ng/ml) (n = 8).B–EAfter 72‐h exposure to normal or HGPA condition, (B) cell apoptosis (n = 8), (C) GSIS (n = 5), (D) insulin content (n = 8), and (E) protein expression of insulin and incretin signaling components (n = 4) were measured in cells with PAX6 overexpression.FPhosphorylated and total Akt abundance were measured in cells with PAX6 overexpression after 15‐min insulin (100 nM) stimulation (n = 4).G, HPhosphorylated and total CREB abundance were measured in cells with PAX6 overexpression after 15‐min (G) Exendin‐4 (10 nM) or (H) GIP (10 nM) stimulation (n = 4).Data information: Each n represents an independent biological replicate (A–H). Unpaired Student's t‐test (A). One‐way ANOVA (B–E). Two‐way ANOVA (F–H). Data are means +/- SEM. ns, nonsignificant.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37933577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLP-1R Antibody - BSA Free

Western Blot: GLP-1R Antibody - BSA Free [NLS1205] -

Beta cell‐specific PAX6 replenishment preserves beta cells and ameliorates glucose homeostasis in db/db miceA, B(A) Fasting blood glucose and (B) serum insulin of db/+ or db/db mice at the indicated time points after AAV injection (n = 10).CIPGTT of db/+ or db/db mice with AAV injection (n = 10).DGlucose profiles calculated as AUC (n = 10).ESerum insulin during IPGTT expressed as percent of basal (n = 10).F–H(F) Serum glucagon, (G) islet insulin content, and (H) islet apoptosis of db/+ or db/db mice with AAV injection (n = 8–10).I, JRepresentative immunostaining and quantification showing (I) insulin/Ki67 and (J) insulin/TUNEL signals in pancreatic islets of db/+ or db/db mice with AAV injection (n = 6). Scale bar = 20 μm.KProtein expression of insulin and incretin signaling components in pancreatic islets of db/+ or db/db mice with AAV injection (n = 4).Data information: Each n represents the measurement of a sample from distinct mice (A–K). Unpaired Student's t‐test and Mann–Whitney test (A–C). db/db + AAV‐PAX6 versus db/db + AAV‐Ctrl. Unpaired Student's t‐test (E). db/db + AAV‐PAX6 versus db/db + AAV‐Ctrl. One‐way ANOVA (D, F–K). Data are means +/- SEM. AUC, area under the curve; ns, nonsignificant.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37933577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLP-1R Antibody - BSA Free

Western Blot: GLP-1R Antibody - BSA Free [NLS1205] -

Beta cell‐specific PAX6 replenishment enhances beta cell survival and maintains beta cell identity in human T2D isletsA, B(A) Islet insulin content and (B) islet apoptosis of normal or T2D human islets with AAV transduction (n = 8).C, DRepresentative immunostaining and quantification showing (C) insulin/Ki67 and (D) insulin/TUNEL signals in normal or T2D human islets with AAV transduction (n = 5–6). Scale bar = 20 μm.EProtein expression of insulin and incretin signaling components in normal or T2D human islets with AAV transduction (n = 4).FPhosphorylated and total Akt in human islets after 15‐min insulin (100 nM) stimulation (n = 4).G, HPhosphorylated and total CREB in human islets after 15‐min (G) Exendin‐4 (10 nM) or (H) GIP (10 nM) stimulation (n = 4).Data information: Each n represents an independent biological replicate (A–H). One‐way ANOVA (A–D). One‐way ANOVA and Kruskal–Wallis test (E). (F–H) Two‐way ANOVA. Data are means +/- SEM. ns, nonsignificant.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37933577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GLP-1R Antibody - BSA Free

Western Blot: GLP-1R Antibody - BSA Free [NLS1205] -

PAX6 is essential to preserve human pancreatic beta cellsAEndoC‐ beta H1 cells with PAX6 knockdown were subjected to cell proliferation measurement indexed by BrdU labeling after 72‐h treatment with insulin (100 nM), Exendin‐4 (10 nM), GIP (10 nM), or IGF1 (50 ng/ml) (n = 8–10).B–D(B) Cell apoptosis (n = 8), (C) GSIS (n = 5), and (D) insulin content (n = 10–11) were measured in control and PAX6 knockdown cells.EProtein expression of insulin and incretin signaling components in cells with PAX6 knockdown (n = 4).FPhosphorylated and total Akt abundance in cells with PAX6 knockdown were measured after 15‐min insulin (100 nM) stimulation (n = 4).G, HPhosphorylated and total CREB abundance in cells with PAX6 knockdown were measured after 15‐min (G) Exendin‐4 (10 nM) or (H) GIP (10 nM) stimulation (n = 4).I, JcAMP concentration was measured in cells with PAX6 knockdown after 15‐min (I) Exendin‐4 (10 nM) or (J) GIP (10 nM) stimulation (n = 6–7).Data information: Each n represents an independent biological replicate (A–J). Mann–Whitney test (A). Unpaired Student's t‐test (B–E, I, J). Two‐way ANOVA (F–H). Data are means +/- SEM. ns, nonsignificant.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37933577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GLP-1R Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 32232832)

Immunohistochemistry

10 - 20 ug/mL

Immunohistochemistry-Paraffin

10 - 20 ug/mL

Microarray

reported by customer review

Simple Western

1:50

Western Blot

1 - 2 ug/mL
Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, Retina, separated by Size, antibody dilution of 1:50, apparent MW was 49 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NLS1205 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GLP-1R

GLP1R, a Glucagon Receptor, has been suggested to affect the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. Glucagon-like peptide 1 is an incretin hormone produced by enteroendocrine L-cells in the intestinal mucosa. The hormone is released in response to food intake and plays an important role in maintaining blood glucose homeostasis. Stimulation of the GLP-1R by endogenous hormone induces multiple complementary mechanisms, which together result in a lowering of circulating blood glucose levels. These mechanisms include receptor-mediated enhancement of glucose-induced insulin secretion from pancreatic -cells, inhibition of gastric emptying with a delay in the gastrointestinal resorption of nutrients, inhibition of glucagon secretion, and inhibition of food intake. Desensitization of GLP1R on pancreatic beta-cells is one of the causes of non insulin-dependent diabetes mellitus (NIDDM). GLP1R knockout mice are viable, develop normally, but exhibit increased levels of blood glucose following oral glucose challenge in association with diminished levels of circulating insulin. Recently it has been shown that overexpression of glucagon-like peptide-1 receptor improves learning in rats (During et al. 2003). GLP1R has been reported in pancreas, brain, heart, kidney, lung, and stomach. ESTs have been isolated from kidney and skin libraries.

Long Name

Glucagon-like Peptide 1 Receptor

Alternate Names

GLP1R

Entrez Gene IDs

2740 (Human)

Gene Symbol

GLP1R

UniProt

P43220, O35659 (Mouse)

Additional GLP-1R Products

Product Documents for GLP-1R Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLP-1R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLP-1R Antibody - BSA Free

Customer Reviews for GLP-1R Antibody - BSA Free (1)

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  • GLP-1R Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    GLP-1R Antibody - BSA Free NLS1205

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Protocols

View specific protocols for GLP-1R Antibody - BSA Free (NLS1205):


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 degrees C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.


Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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