Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1055220
View all 5-HT2A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the amino acids 1-75 in the N-term extracellular domain of the Serotonin Receptor 5-HT2A (HTR2A).
Accession # P28223

Specificity

Detects human HTR2A in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human 5-HT2A Antibody

Detection of 5-HT2A in NS0 mouse myeloma cell line transfected (positive).

5-HT2A was detected in immersion fixed NS0 mouse myeloma cell line transfected (positive) cells and absent in NS0 mouse myeloma cell line wild type (negative) cells using Mouse Anti-Human 5-HT2A Monoclonal Antibody (Catalog # MAB112501) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of 5-HT2A in HEK293 cells transfected with Human HT2A and eGFP vs irrelevant cells by Flow Cytometry.

HEK293 cells transfected with Human HT2A and eGFP vs irrelevant were stained with eGFP and Mouse Anti-Human 5-HT2A Monoclonal Antibody (A, B) (Catalog # MAB112501) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human 5-HT2A Antibody

Application
Recommended Usage

Flow Cytometry

0.5 µg/106 cells
Sample: HEK293 cells transfected with Human HT2A and eGFP vs irrelevant

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NS0 mouse myeloma cell line transfected (positive) and NS0 mouse myeloma cell line wild type (negative) cells

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5-HT2A

Human serotonin receptor HTR2A, also known as 5-HT2A or HTR2, is a G-protein coupled receptor (GPCR) for 5-hydroxytryptamine (serotonin). The 5-HT2A receptor is mainly a cell surface receptor with several intracellular locations. Human -5-HT2A is the main excitatory receptor subtype among the GPCRs for serotonin but an inhibitory effect on the visual cortex and the orbitofrontal cortex has also been described. This receptor was first noted for its importance as a target of serotonergic psychedelic drugs such as LSD and psilocybin mushrooms.

Long Name

5-Hydroxytryptamine Receptor 2A

Alternate Names

5HT2A, HTR2A

Entrez Gene IDs

3356 (Human); 15558 (Mouse); 29595 (Rat)

Gene Symbol

HTR2A

UniProt

P28223 (Human)

Additional 5-HT2A Products

Product Documents for Human 5-HT2A Antibody

Certificate of Analysis

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Product Specific Notices for Human 5-HT2A Antibody

For research use only

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Protocols

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