Human ABCA1 Antibody

Recombinant Monoclonal Antibody..
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ABCA1 in direct ELISAs and Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1276B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Human ABCA1 synthetic peptide
    Accession # O95477
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ABCA1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human ABCA1 Monoclonal Antibody (Catalog # MAB72071) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ABCA1 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: ABCA1

ABCA1 (ATP-binding cassette, subfamily A member 1; also cholesterol efflux regulatory protein) is a 240-280 kDa member of the ABCA family, ABC transporter superfamily of molecules. It is widely expressed, particularly by macrophages, where it participates in the transport of lipid across cell membranes. Once transported, it is passed on to tethered apoA-I to form HDL. Human ABCA1 is 2261 amino acids (aa) in length. It has up to 15 transmembrane segments with beginning and ending cytoplasmic domains and a large cytoplasmic central region. ABC transporters are found between aa 899-1311 and 1912-2144. Amino acids 2216-2221 are essential for lipid transport, and two intramolecular disulfide bonds are required for activity (Cys75-Cys309 and Cys1463/1465-Cys1477). There are three potential isoform variants. One possesses an alternative start site at Met61, while two others contain 12 aa and 123 aa substitutions for aa 241-2261, respectively. Over aa 1248-1350, human ABCA1 shares 97% aa identity with mouse ABCA1.

  • Long Name:
    ATP-binding Cassette, Sub-family A (ABC1), Member 1
  • Entrez Gene IDs:
    19 (Human); 11303 (Mouse)
  • Alternate Names:
    ABC1; ABC-1; ABC1ATP-binding cassette transporter A1; ABCA1; ATP-binding cassette 1; ATP-binding cassette transporter 1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP; Cholesterol efflux regulatory protein; EC 2.7.7.8; EC 2.8.1.8; EC 3.6.3; EC 3.6.3.41; FLJ14958; HDLDT1; membrane-bound; MGC164864; MGC165011; TGD; TGDATP-binding cassette sub-family A member 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 26  
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG Horseradish Peroxidase-conjugated Antibody

WB HAF008 12  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 1  
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NL557 Affinit Purified PAb

Flow, IHC, ICC NL004 6
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Donkey Anti-Rabbit IgG NL493 Affinity Purified PAb

Flow, IHC, ICC NL006 4
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 2
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 2  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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