Human ABCA1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB72071-100
MAB72071-SP
Detection of Human ABCA1 by Western Blot.
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Human ABCA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ABCA1 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1276B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human ABCA1 synthetic peptide
Accession # O95477
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ABCA1 antibody by Western Blot. View Larger

Detection of Human ABCA1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human ABCA1 Monoclonal Antibody (Catalog # MAB72071) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ABCA1 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABCA1

ABCA1 (ATP-binding cassette, subfamily A member 1; also cholesterol efflux regulatory protein) is a 240-280 kDa member of the ABCA family, ABC transporter superfamily of molecules. It is widely expressed, particularly by macrophages, where it participates in the transport of lipid across cell membranes. Once transported, it is passed on to tethered apoA-I to form HDL. Human ABCA1 is 2261 amino acids (aa) in length. It has up to 15 transmembrane segments with beginning and ending cytoplasmic domains and a large cytoplasmic central region. ABC transporters are found between aa 899-1311 and 1912-2144. Amino acids 2216-2221 are essential for lipid transport, and two intramolecular disulfide bonds are required for activity (Cys75-Cys309 and Cys1463/1465-Cys1477). There are three potential isoform variants. One possesses an alternative start site at Met61, while two others contain 12 aa and 123 aa substitutions for aa 241-2261, respectively. Over aa 1248-1350, human ABCA1 shares 97% aa identity with mouse ABCA1.

Long Name
ATP-binding Cassette, Sub-family A (ABC1), Member 1
Entrez Gene IDs
19 (Human); 11303 (Mouse)
Alternate Names
ABC1; ABC-1; ABC1ATP-binding cassette transporter A1; ABCA1; ATP-binding cassette 1; ATP-binding cassette transporter 1; ATP-binding cassette, sub-family A (ABC1), member 1; CERP; Cholesterol efflux regulatory protein; EC 2.7.7.8; EC 2.8.1.8; EC 3.6.3; EC 3.6.3.41; FLJ14958; HDLDT1; membrane-bound; MGC164864; MGC165011; TGD; TGDATP-binding cassette sub-family A member 1

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Product Specific Notices

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

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