Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 171409
View all ACE/CD143 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ACE
Leu30-Leu1261
Accession # P12821.1

Specificity

Detects recombinant human (rh)ACE in direct ELISAs and Western blots. Does not cross-react with rhACE-2 or rhNeprilysin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human ACE/CD143 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human ACE/CD143 Somatic Form (Catalog # 929-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACE/CD143

ACE, also known as kininase II and CD143 antigen, is a dipeptidyl carboxyl peptidase that contains zinc. It plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor and aldosterone-stimulating peptide.

Long Name

Angiotensin I Converting Enzyme

Alternate Names

CD143, DCP1, Kininase II

Entrez Gene IDs

1636 (Human); 11421 (Mouse)

Gene Symbol

ACE

UniProt

P12821.1

Additional ACE/CD143 Products

Product Documents for Human ACE/CD143 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ACE/CD143 Antibody

For research use only

Citations for Human ACE/CD143 Antibody

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FAQs for Human ACE/CD143 Antibody

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  • Q: Is ACE-1 the same as ACE?

    A: Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

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