Human Activin B beta B subunit Antibody

Catalog # Availability Size / Price Qty
MAB659-SP
MAB659-100
MAB659-500
Hemoglobin Expression Induced by Activin B and Neutralization by Human Activin B Antibody.
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Product Details
Citations (3)
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Reviews (1)

Human Activin B beta B subunit Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin B beta B subunit in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Inhibin B or rhActivin A is observed. In Western blots, approximately 20% cross-reactivity with rhInhibin B is observed and no cross‑reactivity with rhActivin A or recombinant mouse Activin A is observed.
Source
Monoclonal Mouse IgG1 Clone # 146807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Activin B
Gly293-Ala407
Accession # Q53T31
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Activin B beta B subunit (Catalog # 659-AB)
Neutralization
Measured by its ability to neutralize Activin B-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. Schwall, R. H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Activin B beta B subunit.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Hemoglobin Expression Induced by Activin B and Neutralization by Human Activin B Antibody. View Larger

Hemoglobin Expression Induced by Activin B and Neutralization by Human Activin B Antibody. Recombinant Human Activin B beta B subunit (Catalog # 659-AB) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Human Activin B beta B subunit (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Activin B beta B subunit Monoclonal Antibody (Catalog # MAB659). The ND50 is typically 0.6-3.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin B

Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha  subunit and one of the various beta  subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and  Xenopus beta D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta A­‑ beta A), activin B ( beta B­‑ beta B), activin AB ( beta A‑ beta B), inhibin A ( alpha ­‑ beta A) and inhibin B ( alpha ­‑ beta B). At present, little is known about the contribution of the other beta  subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha  subunits share approximately 80% identity. Similarly to other TGF-beta  family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low-affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.

References
  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
Long Name
Inhibin Subunit Beta B
Entrez Gene IDs
3625 (Human); 3625 (Human); 16324 (Mouse); 16324 (Mouse); 25196 (Rat); 25196 (Rat)
Alternate Names
activin AB beta polypeptide; Activin Beta B; Activin beta-B chain; INHBB; inhibin beta B chain; Inhibin Beta B; Inhibin Subunit Beta B; inhibin, beta B (activin AB beta polypeptide); inhibin, beta B; Inhibin, beta-2

Product Datasheets

Citations for Human Activin B beta B subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. An autocrine ActivinB mechanism drives TGF?/Activin signaling in Group 3 medulloblastoma
    Authors: M Morabito, M Larcher, FM Cavalli, C Foray, A Forget, L Mirabal-Or, M Andrianter, S Druillenne, A Garancher, J Masliah-Pl, S Leboucher, A Debalkew, A Raso, O Delattre, S Puget, F Doz, MD Taylor, O Ayrault, F Bourdeaut, A Eychène, C Pouponnot
    EMBO Mol Med, 2019;0(0):e9830.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. An activin receptor IIA ligand trap corrects ineffective erythropoiesis in beta-thalassemia.
    Authors: Dussiot M, Maciel T, Fricot A, Chartier C, Negre O, Veiga J, Grapton D, Paubelle E, Payen E, Beuzard Y, Leboulch P, Ribeil J, Arlet J, Cote F, Courtois G, Ginzburg Y, Daniel T, Chopra R, Sung V, Hermine O, Moura I
    Nat Med, 2014;20(4):398-407.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases.
    Authors: Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z
    J. Neurochem., 2007;103(3):962-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Activin B beta B subunit Antibody
By Anonymous on 03/29/2019
Application: see ppt slide Sample Tested: Recombinant protein Species: Human