Human ADAM12 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY4416-05
Ancillary Products Available
Human ADAM12 ELISA Standard Curve
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human ADAM12 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ADAM12. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human ADAM12 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ADAM12

Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.

Long Name:
A Disintegrin and Metalloprotease-like Domain 12
Entrez Gene IDs:
8038 (Human)
Alternate Names:
a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.-

Citations for Human ADAM12 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Stromal-derived interleukin 6 drives epithelial-to-mesenchymal transition and therapy resistance in esophageal adenocarcinoma
    Authors: EA Ebbing, AP van der Za, A Steins, A Creemers, S Hermsen, R Rentenaar, M Klein, C Waasdorp, GKJ Hooijer, SL Meijer, KK Krishnadat, CJA Punt, MI van Berge, SS Gisbertz, OM van Delden, MCCM Hulshof, JP Medema, HWM van Laarho, MF Bijlsma
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Human
    Sample Types: Serum
  2. ADAM12 is a circulating marker for stromal activation in pancreatic cancer and predicts response to chemotherapy
    Authors: VL Veenstra, H Damhofer, C Waasdorp, LB van Rijsse, MJ van de Vij, F Dijk, HW Wilmink, MG Besselink, OR Busch, DK Chang, PJ Bailey, AV Biankin, HM Kocher, JP Medema, JS Li, R Jiang, DW Pierce, HWM van Laarho, MF Bijlsma
    Oncogenesis, 2018;7(11):87.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human ADAM12 DuoSet ELISA

Average Rating: 4 (Based on 1 Review)

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Human ADAM12 DuoSet ELISA
By Anonymous on 07/02/2019
Application: Sample Tested: EDTA Plasma

We ran human EDTA-plasma samples at a 1:2 dilution for this assay,