Human ADAM12 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human ADAM12 ELISA Standard Curve
Preparation and Storage
Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.
Citations for Human ADAM12 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
Filter your results:
Serum-based measurements of stromal activation through ADAM12 associate with poor prognosis in colorectal cancer
Authors: S Ten Hoorn, C Waasdorp, MGH van Oijen, H Damhofer, A Trinh, L Zhao, LJH Smits, S Bootsma, GW van Pelt, WE Mesker, L Mol, KKH Goey, M Koopman, JP Medema, JB Tuynman, I Zlobec, CJA Punt, L Vermeulen, MF Bijlsma
BMC Cancer, 2022;22(1):394.
Sample Types: Serum
Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
Journal of ovarian research, 2022;15(1):87.
Sample Types: Cell Culture Supernates
Stromal-derived interleukin 6 drives epithelial-to-mesenchymal transition and therapy resistance in esophageal adenocarcinoma
Authors: EA Ebbing, AP van der Za, A Steins, A Creemers, S Hermsen, R Rentenaar, M Klein, C Waasdorp, GKJ Hooijer, SL Meijer, KK Krishnadat, CJA Punt, MI van Berge, SS Gisbertz, OM van Delden, MCCM Hulshof, JP Medema, HWM van Laarho, MF Bijlsma
Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
Sample Types: Serum
ADAM12 is a circulating marker for stromal activation in pancreatic cancer and predicts response to chemotherapy
Authors: VL Veenstra, H Damhofer, C Waasdorp, LB van Rijsse, MJ van de Vij, F Dijk, HW Wilmink, MG Besselink, OR Busch, DK Chang, PJ Bailey, AV Biankin, HM Kocher, JP Medema, JS Li, R Jiang, DW Pierce, HWM van Laarho, MF Bijlsma
Sample Types: Serum
No product specific FAQs exist for this product, however you mayView all ELISA FAQs
DuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human ADAM12 DuoSet ELISA
Average Rating: 4 (Based on 1 Review)
Have you used Human ADAM12 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
We ran human EDTA-plasma samples at a 1:2 dilution for this assay,