Human ADAM12 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human ADAM12 ELISA Standard Curve
Preparation and Storage
Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.
Citations for Human ADAM12 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Stromal-derived interleukin 6 drives epithelial-to-mesenchymal transition and therapy resistance in esophageal adenocarcinoma
Authors: EA Ebbing, AP van der Za, A Steins, A Creemers, S Hermsen, R Rentenaar, M Klein, C Waasdorp, GKJ Hooijer, SL Meijer, KK Krishnadat, CJA Punt, MI van Berge, SS Gisbertz, OM van Delden, MCCM Hulshof, JP Medema, HWM van Laarho, MF Bijlsma
Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
Sample Types: Serum
ADAM12 is a circulating marker for stromal activation in pancreatic cancer and predicts response to chemotherapy
Authors: VL Veenstra, H Damhofer, C Waasdorp, LB van Rijsse, MJ van de Vij, F Dijk, HW Wilmink, MG Besselink, OR Busch, DK Chang, PJ Bailey, AV Biankin, HM Kocher, JP Medema, JS Li, R Jiang, DW Pierce, HWM van Laarho, MF Bijlsma
Sample Types: Serum
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