Human ADAM32 Antibody

Catalog # Availability Size / Price Qty
ADAM32 in MCF‑7 Human Cell Line.
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Product Details
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Human ADAM32 Antibody Summary

Species Reactivity
Detects human ADAM32 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 539220
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ADAM32
Accession # Q8TC27
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
Immersion fixed MCF‑7 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

ADAM32 in MCF‑7 Human Cell Line. ADAM32 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human ADAM32 Monoclonal Antibody (Catalog # MAB5380) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM32

ADAM32 (a disintegrin and metalloprotease domain 32) is a 98 kDa member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771 amino acid (aa) type I transmembrane protein. It contains a 158 aa proregion (aa 17‑174) and a 508 aa extracellular domain (ECD) (aa 175‑682). The ECD contains a nonfunctional metalloprotease domain (aa 186‑383), an integrin‑binding disintegrin region (aa 391‑479), a Cys‑rich segment (aa 480‑502) and an EGF‑like domain (aa 622‑654). In the testis, mature ADAM32 is 98 kDa in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44 kDa product. There are two potential splice events that show a deletion of aa 306‑401 plus a 53 aa substitution for the N‑terminal 46 amino acids. Over aa 17‑476, human ADAM32 is 66% aa identical to mouse ADAM32.

Long Name
A Disintegrin and Metalloproteinase Domain 32
Entrez Gene IDs
203102 (Human); 353188 (Mouse); 361170 (Rat)
Alternate Names
a disintegrin and metalloprotease domain 32; a disintegrin and metalloproteinase domain 32; ADAM 32; ADAM metallopeptidase domain 32; ADAM32; disintegrin and metalloproteinase domain-containing protein 32; FLJ26299; FLJ29004; metalloproteinase 12-like protein

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Reconstitution Buffers

Secondary Antibodies

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