Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498‑653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.
Monoclonal Mouse IgG1 Clone # 143338
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain Asp158-Ser653 Accession # P78325
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human ADAM8 by Western Blot. Western blot shows Recombinant Human ADAM8 aa158-653 and Recombinant Human ADAM8 aa 158-497. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ADAM8 Monoclonal Antibody (Catalog # MAB10311) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ADAM8 aa158-653 at approximately 65 kDa (as indicated). The antibody did not detect ADAM8 aa 158-497, indicating it is specific for aa 498-653. This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).
Yoshiyama, K. et al. (1997) Genomics 41:56.
Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.
A Disintegrin and Metalloprotease-like Domain 8
Entrez Gene IDs:
a disintegrin and metalloproteinase domain 8; ADAM 8; ADAM metallopeptidase domain 8; ADAM8; CD156a antigen; CD156a; CD156human leukocyte differentiation antigen; Cell surface antigen MS2; EC 3.4.24; EC 3.4.24.-; MGC134985; MS2; MS2disintegrin and metalloproteinase domain-containing protein 8
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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