Human ADAM8 aa 498-653 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498‑653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 143338
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain
    Asp158-Ser653
    Accession # P78325
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human ADAM8 aa 158-653 see our available Western blot detection antibodies. Does not detect Recombinant Human ADAM8 aa 158-497.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ADAM8 by Western Blot. Western blot shows Recombinant Human ADAM8 aa158-653 and Recombinant Human ADAM8 aa 158-497. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ADAM8 Monoclonal Antibody (Catalog # MAB10311) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ADAM8 aa158-653 at approximately 65 kDa (as indicated). The antibody did not detect ADAM8 aa 158-497, indicating it is specific for aa 498-653. This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

  • References:
    1. Yoshiyama, K. et al. (1997) Genomics 41:56.
    2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
    3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
    4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
    5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
    6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.
  • Long Name:
    A Disintegrin and Metalloprotease-like Domain 8
  • Entrez Gene IDs:
    101 (Human)
  • Alternate Names:
    a disintegrin and metalloproteinase domain 8; ADAM 8; ADAM metallopeptidase domain 8; ADAM8; CD156a antigen; CD156a; CD156human leukocyte differentiation antigen; Cell surface antigen MS2; EC 3.4.24; EC 3.4.24.-; MGC134985; MS2; MS2disintegrin and metalloproteinase domain-containing protein 8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. miR-720 is a downstream target of an ADAM8-induced ERK signaling cascade that promotes the migratory and invasive phenotype of triple-negative breast cancer cells
    Authors: SG Das, M Romagnoli, ND Mineva, S Barillé-Ni, P Jézéquel, M Campone, GE Sonenshein
    Breast Cancer Res, 2016;18(1):40.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding.
    Authors: Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-Pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, Diaz-Gonzalez F
    J. Immunol., 2007;178(12):8053-63.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
    Authors: Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
    Am. J. Respir. Cell Mol. Biol., 2004;31(4):413-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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