Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 496708
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS10
Phe26-Gly1102
Accession # Q9H324
Phe26-Gly1102
Accession # Q9H324
Specificity
Detects human ADAMTS10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human ADAMTS1, 3, 4, 5, 12, 13, 15, 16, or L2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human ADAMTS10 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS10, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS10, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human ADAMTS10 under non-reducing conditions only
Sample: Recombinant Human ADAMTS10 under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ADAMTS10
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 10
Alternate Names
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 10, ADAM metallopeptidase with thrombospondin type 1 motif, 10, ADAM-TS 10, ADAMTS-10, ADAM-TS10A disintegrin and metalloproteinase with thrombospondin motifs 10, EC 3.4.24.-, EC 3.4.24.82, WMS, zinc metalloendopeptidase
Gene Symbol
ADAMTS10
UniProt
Additional ADAMTS10 Products
Product Documents for Human ADAMTS10 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human ADAMTS10 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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