Human ADAMTSL-1/Punctin Antibody

Catalog #: AF1717 Datasheet / COA / SDS

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AF1717
AF1717-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAMTSL-1/Punctin Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTSL‑1/Punctin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAMTSL‑1/Punctin
Glu29-Ser525
Accession # Q8N6G6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ADAMTSL‑1/Punctin

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAMTSL‑1/Punctin, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTSL-1/Punctin

Punctin is a secreted glycoprotein resembling members of the ADAMTS family although it lacks the propeptide region and the metalloproteinase and disintegrin-like domains. Punctin is expressed in adult skeletal muscle and may have important functions in the extracellular matrix. Alternative splicing of the Punctin transcript results in three isoforms. Isoform 1 is the longest and contains the entire isoform 2. Isoform 3 has the same sequence as isoforms 1 and 2 until residue 361, but a unique C-terminal end of 78 amino acids.

Long Name

ADAMTS-like Protein 1

Entrez Gene IDs

92949 (Human)

Alternate Names

ADAM-TS related protein 1; ADAMTSL1; ADAMTSL-1; ADAMTS-like 1; ADAMTS-like protein 1; ADAMTSR1MGC118805; C9orf94; chromosome 9 open reading frame 94; DKFZp686L03130; FLJ35283; FLJ41032; FLJ46891; MGC118803; MGC40193; PUNCTIN; Punctin-1