Human Afamin Antibody

Catalog # Availability Size / Price Qty
Detection of Human Afamin by Western Blot.
1 Image
Product Details
Supplemental Products

Human Afamin Antibody Summary

Species Reactivity
Detects human Afamin in ELISA and Western blot.
Monoclonal Mouse IgG1 Clone # 883113
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human Afamin
Accession # P43652
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Afamin antibody by Western Blot. View Larger

Detection of Human Afamin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Afamin Monoclonal Antibody (Catalog # MAB8065) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Afamin at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Afamin

Afamin, also known as AFM or alpha -Albumin, is a secreted monomeric glycoprotein member of the Alb/albumin family of molecules. Although its MW in SDS-Page ranges from 84-88 kDa, MALDI-TOF analysis yields a likely more correct value of 70-75 kDa. It is expressed by hepatocytes, CNS endothelial cells and osteoclasts, and circulates in the blood at low μg/mL concentrations. Afamin is known to bind and transport vitamin E, particularly under conditions where lipoprotein is limited. This is likely to be important in follicular fluid and CSF. It also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rational for the observation that bone formation often follows bone resorption. Mature human Afamin is 578 amino acids (aa) in length (aa 22-599). It contains three consecutive albumin domains (aa 36-206, 211-403 and 404-599) that contain a characteristic 5 or 6 intrachain disulfide bonds. Full-length human Afamin shares 67% aa sequence identity with mouse Afamin.

Entrez Gene IDs
173 (Human); 280662 (Mouse); 282708 (Rat)
Alternate Names
Afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin; MGC125338; MGC125339

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Afamin Antibody

There are currently no reviews for this product. Be the first to review Human Afamin Antibody and earn rewards!

Have you used Human Afamin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review