Human Akt Pan Specific Antibody

Catalog # Availability Size / Price Qty
AF2055
AF2055-SP
Detection of Human Akt by Western Blot.
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Human Akt Pan Specific Antibody Summary

Species Reactivity
Human
Specificity
Detects human Akt1, Akt2, and Akt3 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Akt synthetic peptide
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Accession # P31749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
20 µg/mL
HDLM‑2 human Hodgkin's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Akt antibody by Western Blot. View Larger

Detection of Human Akt by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and KG-1 human myeloid leukemia cell lines. PVDF membrane was probed with 0.1 µg/mL of Human Akt Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Akt Pan Specific, at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt at approximately 60 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Akt Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt

Rabbit antibodies were raised against a synthetic peptide corresponding to amino acids 75‑90 of human v-akt murine thymoma viral oncogene homolog 1 (Akt1), also known as protein kinase B alpha (PKB alpha ) and RAC-alpha serine/threonine kinase (RAC-PK-alpha ). In addition, this peptide sequence is identical to amino acids 75‑90 of human Akt2 (PKB beta, RAC-PK-beta ), and amino acids 74‑89 of human Akt3 (PKB gamma, RAC-PK-gamma ).

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

Product Datasheets

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Reviews for Human Akt Pan Specific Antibody

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Human Akt Pan Specific Antibody
By Maria Russo on 04/28/2017
Application: Western Blot Sample Tested: Human Chronic Lymphocytic Cell Line Species: Human

30 microg cell homogenate/line
Gel SDS/Page 4-12% BisTris Novex Invitrogen.
Western Blot on PVDF membrane.
Blocking Reagent: 5% non fat dry milk/T-TBS
Anti AKT1-2-3 (R& D System cat. N. AF2055)
1:1250 in 3% BSA/PBS o.n. 4°C
Anti pAKTser473 Cell Signalling 1:1250 in 3%
BSA/PBS o.n. 4°C.
Anti horsadish peroxidase linked II antibody against
Rabbit 1:20.000 (GE Healthcare) in T-TBS 90 min r.t.
ECL Prime Amersham (GE Healhtcare).