Human Akt Pan Specific Antibody

Catalog # Availability Size / Price Qty
Human Akt Pan Specific Antibody in Western Blot
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Product Details
Supplemental Products

Human Akt Pan Specific Antibody Summary

Species Reactivity
Detects human Akt1, Akt2, and Akt3 in Western blots.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Akt synthetic peptide
Accession # P31749
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Akt by Western Blot. View Larger

Detection of Human Akt by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and KG-1 human myeloid leukemia cell lines. PVDF membrane was probed with 0.1 µg/mL of Human Akt Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Akt Pan Specific, at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt

Rabbit antibodies were raised against a synthetic peptide corresponding to amino acids 75‑90 of human v-akt murine thymoma viral oncogene homolog 1 (Akt1), also known as protein kinase B alpha (PKB alpha ) and RAC-alpha serine/threonine kinase (RAC-PK-alpha ). In addition, this peptide sequence is identical to amino acids 75‑90 of human Akt2 (PKB beta, RAC-PK-beta ), and amino acids 74‑89 of human Akt3 (PKB gamma, RAC-PK-gamma ).

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

Product Datasheets


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