Human alpha 1-Acid Glycoprotein DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human alpha 1-Acid Glycoprotein ELISA Standard Curve
Preparation and Storage
Background: alpha 1-Acid Glycoprotein
Alpha 1-acid Glycoprotein (AGP), also known as Orosomucoid, is a Lipocalin family acute-phase protein that is produced by the liver in response to inflammation and infection. Its serum concentration can increase 2-5 fold, making it one of the predominant proteins in serum. AGP functions as a carrier protein for many basic and neutral lipophilic drugs including steroid hormones. It can inhibit neutrophil migration in sepsis, exert a protective effect in severe bacterial infection, and inhibit platelet aggregation. The glycosylation pattern of alpha 1-Acid Glycoprotein influences its anti-inflammatory properties and is associated with various pathophysiological states.
Citation for Human alpha 1-Acid Glycoprotein DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation
Authors: M Jo, JH Kim, G Song, M Seo, E Mi Hwang, K Suk
J. Neurosci, 2017;0(0):.
Sample Types: Plasma
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