Alpha 1-acid Glycoprotein (AGP), also known as Orosomucoid, is a Lipocalin family acute-phase protein that is produced by the liver in response to inflammation and infection. Its serum concentration can increase 2-5 fold, making it one of the predominant proteins in serum. AGP functions as a carrier protein for many basic and neutral lipophilic drugs including steroid hormones. It can inhibit neutrophil migration in sepsis, exert a protective effect in severe bacterial infection, and inhibit platelet aggregation. The glycosylation pattern of alpha 1-Acid Glycoprotein influences its anti-inflammatory properties and is associated with various pathophysiological states.
Human alpha 1-Acid Glycoprotein DuoSet ELISA
R&D Systems | Catalog # DY3694
Loading...
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
15.6-1000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human alpha 1-Acid Glycoprotein DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Loading...
Product Summary for Human alpha 1-Acid Glycoprotein DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human alpha 1-Acid Glycoprotein. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human alpha 1-Acid Glycoprotein DuoSet ELISA
Human alpha 1-Acid Glycoprotein ELISA Standard Curve
Kit Contents for Human alpha 1-Acid Glycoprotein DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: alpha 1-Acid Glycoprotein
Alternate Names
AGP, AGP1, AGPA, alpha 1Acid Glycoprotein, ORM1, Orosomucoid
Gene Symbol
ORM1
Additional alpha 1-Acid Glycoprotein Products
Product Documents for Human alpha 1-Acid Glycoprotein DuoSet ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human alpha 1-Acid Glycoprotein DuoSet ELISA
For research use only
Related Research Areas
Citations for Human alpha 1-Acid Glycoprotein DuoSet ELISA
Powered by Bioz
Customer Reviews for Human alpha 1-Acid Glycoprotein DuoSet ELISA (4)
4.8 out of 5
4 Customer Ratings
Have you used Human alpha 1-Acid Glycoprotein DuoSet ELISA?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
4 of
4 reviews
Showing All
Filter By:
-
Sample Tested: EDTA PlasmaVerified Customer | Posted 11/12/2020For those who want to use this DuoSet kit in human EDTA plasma. We introduced substantial modifications to the protocol described in the product insert. The modifications are derived from the exhaustive testing carried out on Human plasma samples, for which “normal levels” of AGP range between 0.4-2mg/ml whilst the DuoSet ELISA kit DY3694 has a detection range of 15.6 - 1,000 pg/ml, which requires a sample dilution of 1: 2 x106. Also additional washes required.
-
Sample Tested: Purified StandardVerified Customer | Posted 03/30/2019
-
Sample Tested: Purified Standard and UrineVerified Customer | Posted 10/09/2018
-
Sample Tested: Purified StandardVerified Customer | Posted 09/11/2018Followed the protocol as the company provided.
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...