Human alpha 1-Acid Glycoprotein DuoSet ELISA

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Human alpha 1-Acid Glycoprotein ELISA Standard Curve
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Product Details
Citations (2)
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Reviews (4)

Human alpha 1-Acid Glycoprotein DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human alpha 1-Acid Glycoprotein. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human alpha 1-Acid Glycoprotein ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha 1-Acid Glycoprotein

Alpha 1-acid Glycoprotein (AGP), also known as Orosomucoid, is a Lipocalin family acute-phase protein that is produced by the liver in response to inflammation and infection. Its serum concentration can increase 2-5 fold, making it one of the predominant proteins in serum. AGP functions as a carrier protein for many basic and neutral lipophilic drugs including steroid hormones. It can inhibit neutrophil migration in sepsis, exert a protective effect in severe bacterial infection, and inhibit platelet aggregation. The glycosylation pattern of alpha 1-Acid Glycoprotein influences its anti-inflammatory properties and is associated with various pathophysiological states.

Entrez Gene IDs:
5004 (Human); 18405 (Mouse); 24614 (Rat)
Alternate Names:
AGP 1; AGP; AGP1; AGP1alpha-1-acid glycoprotein 1; AGPA; AGP-A; alpha 1Acid Glycoprotein; alpha 1-Acid Glycoprotein; OMD 1; ORM; ORM1; orosomucoid 1; Orosomucoid; orosomucoid-1

Citations for Human alpha 1-Acid Glycoprotein DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Plasma and fecal zonulin are not altered by a high green leafy vegetable dietary intervention: secondary analysis of a randomized control crossover trial
    Authors: AJ Riviere, KS Smith, MN Schaberg, MW Greene, AD Frugé
    BMC gastroenterology, 2022;22(1):184.
    Species: Human
    Sample Types: Serum
  2. Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation
    Authors: M Jo, JH Kim, G Song, M Seo, E Mi Hwang, K Suk
    J. Neurosci, 2017;0(0):.
    Species: Human
    Sample Types: Plasma


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Reviews for Human alpha 1-Acid Glycoprotein DuoSet ELISA

Average Rating: 4.8 (Based on 4 Reviews)

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Human alpha 1-Acid Glycoprotein DuoSet ELISA
By Anonymous on 11/12/2020
Sample Tested: EDTA Plasma

For those who want to use this DuoSet kit in human EDTA plasma. We introduced substantial modifications to the protocol described in the product insert. The modifications are derived from the exhaustive testing carried out on Human plasma samples, for which “normal levels” of AGP range between 0.4-2mg/ml whilst the DuoSet ELISA kit DY3694 has a detection range of 15.6 - 1,000 pg/ml, which requires a sample dilution of 1: 2 x106. Also additional washes required.

Human alpha 1-Acid Glycoprotein DuoSet ELISA
By Anonymous on 03/30/2019
Sample Tested: Purified Standard

Human alpha 1-Acid Glycoprotein DuoSet ELISA
By Anonymous on 10/09/2018
Sample Tested: Purified Standard,Urine

Human alpha 1-Acid Glycoprotein DuoSet ELISA
By Anonymous on 09/11/2018
Sample Tested: Purified Standard

Followed the protocol as the company provided.