Human alpha 1-Acid Glycoprotein DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human alpha 1-Acid Glycoprotein ELISA Standard Curve
Preparation and Storage
Background: alpha 1-Acid Glycoprotein
Alpha 1-acid Glycoprotein (AGP), also known as Orosomucoid, is a Lipocalin family acute-phase protein that is produced by the liver in response to inflammation and infection. Its serum concentration can increase 2-5 fold, making it one of the predominant proteins in serum. AGP functions as a carrier protein for many basic and neutral lipophilic drugs including steroid hormones. It can inhibit neutrophil migration in sepsis, exert a protective effect in severe bacterial infection, and inhibit platelet aggregation. The glycosylation pattern of alpha 1-Acid Glycoprotein influences its anti-inflammatory properties and is associated with various pathophysiological states.
Citations for Human alpha 1-Acid Glycoprotein DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Plasma and fecal zonulin are not altered by a high green leafy vegetable dietary intervention: secondary analysis of a randomized control crossover trial
Authors: AJ Riviere, KS Smith, MN Schaberg, MW Greene, AD Frugé
BMC gastroenterology, 2022;22(1):184.
Sample Types: Serum
Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation
Authors: M Jo, JH Kim, G Song, M Seo, E Mi Hwang, K Suk
J. Neurosci, 2017;0(0):.
Sample Types: Plasma
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Average Rating: 4.8 (Based on 4 Reviews)
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For those who want to use this DuoSet kit in human EDTA plasma. We introduced substantial modifications to the protocol described in the product insert. The modifications are derived from the exhaustive testing carried out on Human plasma samples, for which “normal levels” of AGP range between 0.4-2mg/ml whilst the DuoSet ELISA kit DY3694 has a detection range of 15.6 - 1,000 pg/ml, which requires a sample dilution of 1: 2 x106. Also additional washes required.