Human alpha -2B Adrenergic R/ADRA2B Antibody

Catalog # Availability Size / Price Qty
MAB10324-100
MAB10324-SP
Human  alpha -2B Adrenergic R/ADRA2B Antibody in Flow Cytometry
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Human alpha -2B Adrenergic R/ADRA2B Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha ‑2B Adrenergic R/ADRA2B in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 491613
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human alpha ‑2B Adrenergic R/ADRA2B
Accession # P18089
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of ADRA2B  in HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP by Flow  Cytometry. View Larger

Detection of ADRA2B in HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2B or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human ADRA2B Monoclonal Antibody (Catalog # MAB10324) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-2B Adrenergic R/ADRA2B

Alpha-2-adrenergic receptors, including ADRA2B, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C that have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. ADRA2B was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of ADRA2B was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity.

Long Name
Alpha-2B Adrenergic Receptor
Entrez Gene IDs
151 (Human); 11552 (Mouse); 24174 (Rat)
Alternate Names
a-2B AdrenergicR; ADRA2B adrenergic, alpha-2B-, receptor; ADRA2B; ADRA2L1; ADRA2L1adrenergic receptor alpha 2B; ADRA2RL1; ADRARL1; adrenergic, alpha-2B-, receptor; Alpha-2 adrenergic receptor subtype C2; alpha-2-adrenergic receptor-like 1; alpha-2B Adrenergic R; alpha-2B adrenergic receptor; alpha2B AdrenergicR; Alpha-2B adrenoceptor; Alpha-2B adrenoreceptor; alpha-2B-adrenergic receptor; ALPHA2BAR; alpha-2BAR; G-protein coupled receptor

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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