Human alpha -2B Adrenergic R/ADRA2B Antibody

Catalog #: MAB10324 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10324-100
MAB10324-SP

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Detection of ADRA2B in HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP by Flow Cytometry.

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All alpha-2B Adrenergic R/ADRA2B Antibodies

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Human alpha -2B Adrenergic R/ADRA2B Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ‑2B Adrenergic R/ADRA2B in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 491613

Purification

Protein A or G purified from ascites

Immunogen

NS0 mouse myeloma cell line transfected with human alpha ‑2B Adrenergic R/ADRA2B
Accession # P18089

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ADRA2B in HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2B or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human ADRA2B Monoclonal Antibody (Catalog # MAB10324) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-2B Adrenergic R/ADRA2B

Alpha-2-adrenergic receptors, including ADRA2B, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C that have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. ADRA2B was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of ADRA2B was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity.

Long Name

Alpha-2B Adrenergic Receptor

Entrez Gene IDs

151 (Human); 11552 (Mouse); 24174 (Rat)

Alternate Names

a-2B AdrenergicR; ADRA2B adrenergic, alpha-2B-, receptor; ADRA2B; ADRA2L1; ADRA2L1adrenergic receptor alpha 2B; ADRA2RL1; ADRARL1; adrenergic, alpha-2B-, receptor; Alpha-2 adrenergic receptor subtype C2; alpha-2-adrenergic receptor-like 1; alpha-2B Adrenergic R; alpha-2B adrenergic receptor; alpha2B AdrenergicR; Alpha-2B adrenoceptor; Alpha-2B adrenoreceptor; alpha-2B-adrenergic receptor; ALPHA2BAR; alpha-2BAR; G-protein coupled receptor

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