Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 214107
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Human umbilical cord serum-derived alpha -Fetoprotein/AFP

Specificity

Detects purified human alpha -Fetoprotein/AFP in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human alpha-Fetoprotein/AFP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human alpha -Fetoprotein/AFP under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.

References

  1. Matsumura, M. et al. (2001) Hepatol. Res. 20:84
  2. Deutsch, H.F. et al. (2000) Tumor Biol. 21:267

    Alternate Names

    AFP, DSCAM2

    Entrez Gene IDs

    174 (Human); 11576 (Mouse); 24177 (Rat)

    Gene Symbol

    AFP

    Additional alpha-Fetoprotein/AFP Products

    Product Documents for Human alpha-Fetoprotein/AFP Antibody

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

    Download SDS

    Product Specific Notices for Human alpha-Fetoprotein/AFP Antibody

    For research use only

    Citations for Human alpha-Fetoprotein/AFP Antibody

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