Human alpha-L-Iduronidase/IDUA Antibody (452619)

R&D Systems | Catalog # MAB4119

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 452619
View all alpha-L-Iduronidase/IDUA Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human alpha ‑L‑Iduronidase/IDUA
Ala26-Pro653 (Ala26Thr)
Accession # P35475.2

Specificity

Detects human alpha ‑L‑Iduronidase/IDUA in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human alpha-L-Iduronidase/IDUA Antibody (452619)

Detection of Human a-L-Iduronidase/IDUA antibody by Western Blot.

Detection of Human alpha ‑L‑Iduronidase/IDUA by Western Blot.

Western blot shows lysates of human kidney tissue and IMR-90 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a-L-Iduronidase/IDUA Monoclonal Antibody (Catalog # MAB4119) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-L-Iduronidase/IDUA at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human alpha-L-Iduronidase/IDUA Antibody (452619)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human kidney tissue and IMR‑90 human lung fibroblast cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB4119 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-L-Iduronidase/IDUA

alpha -L-Iduronidase encoded by the IDUA gene is an important enzyme required for the lysosomal degradation of glycosaminoglycans (GAGs). It hydrolyzes the non‑reducing terminal alpha -L-iduronic acid residues in GAGs including dermatan sulfate and heparan sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I) (1). MPS I causes progressive cellular, tissue and organ damage, and several clinical studies using enzyme replacement therapy have shown promising benefits (2). The sequence of human IDUA shares 79% amino acid identity with mouse IDUA.

References

  1. Scott, H.S. et al. (1995) Hum. Mutat. 6:288.
  2. Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489.

Alternate Names

alphaLIduronidase, IDA, IDUA

Entrez Gene IDs

3425 (Human); 15932 (Mouse); 360904 (Rat); 102116889 (Cynomolgus Monkey)

Gene Symbol

IDUA

UniProt

P35475.2

Additional alpha-L-Iduronidase/IDUA Products

Product Documents for Human alpha-L-Iduronidase/IDUA Antibody (452619)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha-L-Iduronidase/IDUA Antibody (452619)

For research use only

Citations for Human alpha-L-Iduronidase/IDUA Antibody (452619)

Customer Reviews for Human alpha-L-Iduronidase/IDUA Antibody (452619) (2)

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  • Human alpha -L-Iduronidase/IDUA Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T cell lysate
    Species: Human
    Verified Customer | Posted 07/07/2020
    Western blot of HEK wild type versus HEK over expressing IDUA.
    Antibody worked as expected at manufacturer recommended concentration. Would order again.
    Human alpha-L-Iduronidase/IDUA Antibody (452619) MAB4119
  • Human alpha -L-Iduronidase/IDUA Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Monoclonal Mouse IgG1 Clone
    Species: Mouse
    Verified Customer | Posted 09/01/2017
    We used this antibody for an ELISA in order to detect human Iduronidase as a positive control for our tests. It worked perfectly at a working concentration of 2ug/mL.

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