Human alpha -Taxilin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human alpha ‑Taxilin in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 812901
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human alpha ‑Taxilin
    Met1-Lys162
    Accession # P40222
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human alpha ‑Taxilin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human alpha ‑Taxilin Monoclonal Antibody (Catalog # MAB5575) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for alpha ‑Taxilin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Taxilin

alpha ‑Taxilin (TXLN‑ alpha ; also p75 [in rat] and IL‑14 alpha ) is the founding member of the taxilin family of proteins. Although its predicted MW is 62 kDa, it runs anomalously at 74‑76 kDa in SDS‑PAGE. alpha ‑Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha ‑Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha ‑Taxilin also regulates the placement of growing protein chains. Human alpha ‑Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled‑coil region between aa 186‑491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL‑14 alpha. Over aa 1‑162, human alpha ‑Taxilin shares 81% aa identity with mouse alpha ‑Taxilin.

  • Entrez Gene IDs:
    200081 (Human); 109658 (Mouse)
  • Alternate Names:
    alphaTaxilin; alpha-Taxilin; DKFZp451J0118; IL14; IL-14; interleukin 14; MGC118870; MGC118871; RP4-622L5.4; taxilin alpha; TXLN; TXLNA
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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