|Aminopeptidase O/ONPEP in Human Kidney. Aminopeptidase O/ONPEP was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Aminopeptidase O/ONPEP Monoclonal Antibody (Catalog # MAB4647) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Aminopeptidase O (AP-O; also C9orf3) is a 93 kDa (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N terminal Arg and Asn residues. Human Aminopeptidase O is 819 amino acids in length. It contains one catalytic domain (aa 167‑589) with a Zn-binding site (His479-His483) and a C terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455‑553; a second has an 11 aa substitution for the C terminal 265 amino acids; and a third shows both a deletion of aa 455‑553 and an 11 aa substitution for the C-terminal 46 amino acids. Full length human Aminopeptidase O is 76% aa identical to mouse Aminopeptidase O.
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