Human Angiostatin Antibody

R&D Systems | Catalog # AF226

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Parasite - Plasmodium falciparum

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation, ELISA Development, Immunogold Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Angiostatin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Angiostatin Kringle 1-3
Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
Accession # P00747

Specificity

Detects human Angiostatin in direct ELISAs and Western blots. This antibody will also recognize human plasminogen.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Angiostatin Antibody

Detection of Angiostatin by Western Blot

Detection of Angiostatin by Western Blot

ALPL deficiency induces excessive anti-angiogenic exosomes secretion in hBMMSCs. (A-B) Western Blot analysis showed PDGFBB, VEGF, Angiostatin and Endostatin expression in Nor and HPP exosomes. (C) ELISA analysis of PDGFBB, VEGF, Angiostatin and Endostatin levels in the Nor and HPP group. (D) Western Blot showed exosomes from Nor and HPP hBMMSCs expressed CD9 and TSG101. Exosomes proteins from equal volumes of culture supernatant of Nor and HPP hBMMSCs were loaded for Western Blot. (E) Exosomes volumes derived from Nor and HPP hBMMSCs were detected by exosomes ELISA complete kit. (F-G) The intracellular expression of CD9 and CD81 were assessed by Western Blot analysis. (H) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in Nor and HPP hBMMSCs. Scale bar, 10 μm. (I) Western Blot showed exosomes from HPP and HPP + pLenti-ALPL hBMMSCs expressed CD9 and TSG101. Exosomes proteins from equal volumes of culture supernatant of HPP and HPP + pLenti-ALPL hBMMSCs were loaded for Western Blot. (J) Exosomes volumes derived from HPP and HPP + pLenti-ALPL hBMMSCs were detected by exosomes ELISA complete kit. (K-L) The intracellular expression of CD9 and CD81 were assessed by Western Blot analysis. (M) Immunofluorescent staining showed CD9 and CD81-positive labeled exosomal proteins localized in HPP and HPP + pLenti-ALPL hBMMSCs. Scale bar, 10 μm. All results were generated in three independent experiments. Data were shown as mean ± standard deviation (SD); *P < 0.05; **P < 0.01; ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-02…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Angiostatin Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Angiostatin Kringle 1-3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiostatin

Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin.  Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810).  It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots.  Plasminogen is also cleaved by MMPs which generate multiple fragments.  Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a downregulation of Bcl2, and an upregulation of thrombospondin-1.  Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.  

Alternate Names

HAE4

Entrez Gene IDs

5340 (Human); 18815 (Mouse)

Gene Symbol

PLG

UniProt

P00747

Additional Angiostatin Products

Product Documents for Human Angiostatin Antibody

Certificate of Analysis

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Product Specific Notices for Human Angiostatin Antibody

For research use only

Citations for Human Angiostatin Antibody

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