Detection of Human BAP31 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BAP31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAP31 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BAP31 (BCR-associated protein of 31 kDa) is a 29 kDa member of the BCAP29/BCAP31 family of proteins. It is ubiquitously expressed, resides in the ER and serves multiple functions. It is known to act as a cargo receptor for ER export of transmembrane (TM) proteins, and serve as a component of the ER quality control compartment, cycling between the peripheral and juxtanuclear ER. Human BAP31 is a 246 amino acid (aa), 3-TM domain containing protein. Amino acids 1‑6 are lumenal, 124‑246 are cytoplasmic, and 7‑27, 44‑64 and 103‑123 constitute TM segments. BAP31 forms homodimers and heterodimers with BAP29. Cleavage between Asp164Gly165 and Asp238Gly239 generates 20 kDa and 27 kDa fragments, respectively. There is an alternate start site 67 aa upstream from the standard start site. Over aa 165‑246, human BAP31 shares 82% aa identity with mouse BAP31.
B-cell Receptor-associated Protein 31
Entrez Gene IDs:
10134 (Human); 27061 (Mouse); 293852 (Rat)
6C6-Ag; BAP31; Bap31,6C6-AG; BAP31DXS1357ECDM; BCAP31; B-cell receptor-associated protein 31; BCR-associated protein 31; BCR-associated protein Bap31,6C6-AG tumor-associated antigen; p28 Bap31,6C6-Ag; p28; Protein CDM
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