Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BAP31 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BAP31
Gly165-Glu246
Accession # P51572

Specificity

Detects human BAP31 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BAP31 Antibody

Detection of Human BAP31 antibody by Western Blot.

Detection of Human BAP31 by Western Blot.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BAP31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAP31 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human BAP31 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAP31

BAP31 (BCR-associated protein of 31 kDa) is a 29 kDa member of the BCAP29/BCAP31 family of proteins. It is ubiquitously expressed, resides in the ER and serves multiple functions. It is known to act as a cargo receptor for ER export of transmembrane (TM) proteins, and serve as a component of the ER quality control compartment, cycling between the peripheral and juxtanuclear ER. Human BAP31 is a 246 amino acid (aa), 3-TM domain containing protein. Amino acids 1‑6 are lumenal, 124‑246 are cytoplasmic, and 7‑27, 44‑64 and 103‑123 constitute TM segments. BAP31 forms homodimers and heterodimers with BAP29. Cleavage between Asp164Gly165 and Asp238Gly239 generates 20 kDa and 27 kDa fragments, respectively. There is an alternate start site 67 aa upstream from the standard start site. Over aa 165‑246, human BAP31 shares 82% aa identity with mouse BAP31.

Long Name

B-cell Receptor-associated Protein 31

Alternate Names

6C6-Ag, BCAP31

Entrez Gene IDs

10134 (Human); 27061 (Mouse); 293852 (Rat)

Gene Symbol

BCAP31

UniProt

P51572 (Human)

Additional BAP31 Products

Product Documents for Human BAP31 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BAP31 Antibody

For research use only

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