Human BAP31 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human BAP31 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human BAP31
    Gly165-Glu246
    Accession # P51572
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human BAP31 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BAP31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAP31 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAP31

BAP31 (BCR-associated protein of 31 kDa) is a 29 kDa member of the BCAP29/BCAP31 family of proteins. It is ubiquitously expressed, resides in the ER and serves multiple functions. It is known to act as a cargo receptor for ER export of transmembrane (TM) proteins, and serve as a component of the ER quality control compartment, cycling between the peripheral and juxtanuclear ER. Human BAP31 is a 246 amino acid (aa), 3-TM domain containing protein. Amino acids 1‑6 are lumenal, 124‑246 are cytoplasmic, and 7‑27, 44‑64 and 103‑123 constitute TM segments. BAP31 forms homodimers and heterodimers with BAP29. Cleavage between Asp164Gly165 and Asp238Gly239 generates 20 kDa and 27 kDa fragments, respectively. There is an alternate start site 67 aa upstream from the standard start site. Over aa 165‑246, human BAP31 shares 82% aa identity with mouse BAP31.

  • Long Name:
    B-cell Receptor-associated Protein 31
  • Entrez Gene IDs:
    10134 (Human); 27061 (Mouse); 293852 (Rat)
  • Alternate Names:
    6C6-Ag; BAP31; Bap31,6C6-AG; BAP31DXS1357ECDM; BCAP31; B-cell receptor-associated protein 31; BCR-associated protein 31; BCR-associated protein Bap31,6C6-AG tumor-associated antigen; p28 Bap31,6C6-Ag; p28; Protein CDM
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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