Human beta -2 Adrenergic R/ADRB2 Antibody

Catalog # Availability Size / Price Qty
MAB100401-100
MAB100401-SP
Detection of  beta ‑2 Adrenergic R/ADRB2 in HEK293 Human Cell Line Transfected with Human  beta ‑2 Adrenergic R/ADRB2 and eGFP by Flow Cytometry.
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Human beta -2 Adrenergic R/ADRB2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta ‑2 Adrenergic R/ADRB2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 586107
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human beta ‑2 Adrenergic R/ADRB2
Accession # P07550
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of  beta ‑2 Adrenergic R/ADRB2 in HEK293 Human Cell Line Transfected with Human  beta ‑2 Adrenergic R/ADRB2 and eGFP by Flow Cytometry. View Larger

Detection of beta ‑2 Adrenergic R/ADRB2 in HEK293 Human Cell Line Transfected with Human beta ‑2 Adrenergic R/ADRB2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human beta ‑2 Adrenergic R/ADRB2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta ‑2 Adrenergic R/ADRB2 Monoclonal Antibody (Catalog # MAB100401) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-2 Adrenergic R/ADRB2

Beta-2 adrenoceptor (ADRB2) is a 413 amino acids adrenergic receptor expressed in smooth muscle and metabolic tissues. Beta-adrenergic receptors belong to the seven-transmembrane domain/GPCR family of receptors and mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Activation of ADRB2 induces a decrease in gastrointestinal motility, bronchodilation, vasodilation in skeletal and cardiac muscle, and glycogenolysis in liver. Agonists of ADRB2 are most widely used for the treatment of asthma. In complex with beta-arrestin-1 and c-src, the beta-2 adrenergic receptor activates MAP kinases ERK1(MAPK3) and ERK2 (MAPK1). Expression of the beta-2 adrenoceptor has been reported in adipose, blood, brain, heart, lung, nose, pancreas, skeletal muscle, skin, and vessel.

Long Name
Beta-2 Adrenergic Receptor
Entrez Gene IDs
154 (Human); 11555 (Mouse); 24176 (Rat)
Alternate Names
ADRB2; ADRB2R; ADRBR; adrenergic, beta-2-, receptor, surface; B2AR; B2ARADRB2R; BAR; beta-2 Adrenergic R; beta-2 adrenergic receptor; beta2 AdrenergicR; beta-2 AdrenergicR; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; catecholamine receptor

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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