beta ‑Actin in HeLa Human Cell Line.
beta ‑Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human beta ‑Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
up the most abundant protein in many eukaryotic cell types. It polymerizes to
form microfilaments that have an array of functions, including regulating
contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology,
and providing structural support. At least six types of Actin, falling into
three categories, exist in higher eukaryotes. The three types, separated based on
isoelectric point, include alpha ( alpha
-Skeletal, alpha -Cardiac, alpha -Smooth
Muscle), beta ( beta -Non-muscle) and gamma ( gamma -Smooth Muscle, gamma -non-muscle) isoforms. Non-muscle
beta - and gamma -actin are also known as cytoplasmic actin.
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