Human beta -Actin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human beta ‑Actin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 937210
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Peptide containing a sequence at the N-terminus of human beta ‑Actin
    Accession # P60709
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
beta ‑Actin in HeLa Human Cell Line.
beta ‑Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse
Anti-Human beta ‑Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Actin
Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and  gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.
  • Entrez Gene IDs:
    60 (Human); 11461 (Mouse); 81822 (Rat)
  • Alternate Names:
    ACTB; actin, beta; actin, cytoplasmic 1; Beta Actin; beta cytoskeletal actin; betaActin; beta-Actin; BRWS1; PS1TP5-binding protein 1; PS1TP5BP1
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