Human beta -Actin Antibody

Catalog # Availability Size / Price Qty
MAB8969
MAB8969-SP
beta ‑Actin  in HeLa Human Cell Line.
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Product Details
Citations (3)
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Human beta -Actin Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta ‑Actin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 937210
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Peptide containing a sequence at the N-terminus of human beta ‑Actin
Accession # P60709
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry beta ‑Actin  in HeLa Human Cell Line. View Larger

beta ‑Actin in HeLa Human Cell Line.
beta ‑Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse
Anti-Human beta ‑Actin Monoclonal Antibody (Catalog # MAB8969) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Actin

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and  gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

Entrez Gene IDs
60 (Human); 11461 (Mouse); 81822 (Rat)
Alternate Names
ACTB; actin, beta; actin, cytoplasmic 1; Beta Actin; beta cytoskeletal actin; betaActin; beta-Actin; BRWS1; PS1TP5-binding protein 1; PS1TP5BP1

Product Datasheets

Citations for Human beta -Actin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway
    Authors: P Shen, J Chen, M Pan
    Mol Med Rep, 2018;18(3):3332-3340.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  2. Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??
    Authors: B Liu, L Xu, X Yu, W Li, X Sun, S Xiao, M Guo, H Wang
    Mol Med Rep, 2018;18(2):1987-1994.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells
    Authors: Y Mao, L Wang, C Xu, S Han
    Oncol Lett, 2018;15(5):6599-6603.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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