Human beta -Defensin 4/2 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human beta -Defensin 4/2 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human beta -Defensin 4/2
    Gly24-Pro64
    Accession # O15263
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human beta-Defensin 4/2
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
beta -Defensin 4/2 in Human Lung. beta -Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human lung using Human beta -Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
beta -Defensin 4/2 in Human Kidney Array. beta -Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human beta -Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Defensin 2
Human BD-2, also known as defensin beta 2 (DEFB-2), is a secreted, 41 amino acid residue anti-microbial protein belonging to the defensin family. Mature defensins are cationic proteins with three intramolecular disulfides and a characteristic triple-stranded beta -sheet. Human BD-2 expression is induced by inflammatory signals in multiple tissues, including airway epithelial cells and keratinocytes. At the amino acid sequence level, human BD-2 shares 98% and 51% homology with the proteins from rhesus monkey and rat, respectively.
  • Long Name:
    Defensin 2 beta
  • Entrez Gene IDs:
    1673 (Human)
  • Alternate Names:
    BD-2; betaDefensin 2; beta-Defensin 2; DEFB2; DEFB-2; DEFB2DEFB102; DEFB4A; DEFB4B; DEFB4beta-defensin 4A; Defensin, beta 2Skin-antimicrobial peptide 1; defensin, beta 4; defensin, beta 4A; HBD-2; SAP-1; SAP1BD-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model.
    Authors: Haisma E, Rietveld M, de Breij A, van Dissel J, El Ghalbzouri A, Nibbering P
    PLoS ONE, 2013;8(12):e82800.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. A Novel Organotypic Dento-Epithelial Culture Model: Effect of Fusobacterium nucleatum Biofilm on B-Defensin-2, -3, and LL-37 Expression.
    Authors: Gursoy UK, Pollanen M, Kononen E, Uitto VJ
    J. Periodontol., 2011;83(2):242-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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