|BIK in Human Lymphoma. BIK was detected in immersion fixed paraffin-embedded sections of human lymphoma tissue using Sheep Anti-Human BIK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5474) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
|Detection of Human BIK by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BIK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BIK at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
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