Human Brachyury Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Brachyury in BG01V Human Embryonic Stem Cells.
1 Image
Product Details
Citations (4)
Supplemental Products

Human Brachyury Antibody Summary

Species Reactivity
Detects human Brachyury in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 1161B
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human Brachyury
Accession # O15178
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Brachyury antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brachyury

Brachyury is a 435 aa T-box family transcription factor.  Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively.  It is required in the early determination and differentiation of mesoderm.  Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.

Entrez Gene IDs
6862 (Human)
Alternate Names
brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

Product Datasheets

You must select a language.


Citations for Human Brachyury Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation
    Authors: AM Drozd, L Mariani, X Guo, V Goitea, NA Menezes, E Ferretti
    International Journal of Molecular Sciences, 2022;23(18):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  3. 7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome
    Authors: H Tomita, KM Hines, JM Herron, A Li, DW Baggett, L Xu
    Elife, 2022;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Combined PDGFR and HDAC Inhibition Overcomes PTEN Disruption in Chordoma.
    Authors: Lee D, Zhang Y, Kassam A, Park M, Gardner P, Prevedello D, Henry S, Horbinski C, Beumer J, Tawbi H, Williams B, Shaffrey M, Egorin M, Abounader R, Park D
    PLoS ONE, 2015;10(8):e0134426.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Brachyury Antibody

There are currently no reviews for this product. Be the first to review Human Brachyury Antibody and earn rewards!

Have you used Human Brachyury Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review