Human Brachyury Antibody

Catalog #: MAB20851
4 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB20851-SP
MAB20851-100

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Brachyury in BG01V Human Embryonic Stem Cells.

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Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Brachyury Antibody Summary

Species Reactivity

Human

Specificity

Detects human Brachyury in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1161B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human Brachyury
Met230-Met435
Accession # O15178

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brachyury

Brachyury is a 435 aa T-box family transcription factor. Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively. It is required in the early determination and differentiation of mesoderm. Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.

Entrez Gene IDs

6862 (Human)

Alternate Names

brachyury protein; Brachyury; MGC104817; Protein T; SAVA; T brachyury (mouse) homolog; T brachyury homolog; T Brachyury Transcription Factor; T, brachyury homolog (mouse); T-Box Transcription Factor T; TBXT; TF; TFT

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Brachyury Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
International Journal of Molecular Sciences, 2022;23(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation
Authors: AM Drozd, L Mariani, X Guo, V Goitea, NA Menezes, E Ferretti
International Journal of Molecular Sciences, 2022;23(18):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF
7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome
Authors: H Tomita, KM Hines, JM Herron, A Li, DW Baggett, L Xu
Elife, 2022;11(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Combined PDGFR and HDAC Inhibition Overcomes PTEN Disruption in Chordoma.
Authors: Lee D, Zhang Y, Kassam A, Park M, Gardner P, Prevedello D, Henry S, Horbinski C, Beumer J, Tawbi H, Williams B, Shaffrey M, Egorin M, Abounader R, Park D
PLoS ONE, 2015;10(8):e0134426.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot