Human Brachyury Antibody Summary
Met230-Met435
Accession # O15178
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Brachyury
Brachyury is a 435 aa T-box family transcription factor. Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively. It is required in the early determination and differentiation of mesoderm. Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.
Product Datasheets
Citation for Human Brachyury Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Combined PDGFR and HDAC Inhibition Overcomes PTEN Disruption in Chordoma.
Authors: Lee D, Zhang Y, Kassam A, Park M, Gardner P, Prevedello D, Henry S, Horbinski C, Beumer J, Tawbi H, Williams B, Shaffrey M, Egorin M, Abounader R, Park D
PLoS ONE, 2015;10(8):e0134426.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Secondary Antibodies
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