Human Brachyury Antibody Summary
Accession # O15178
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Brachyury in BG01V Human Embryonic Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Rabbit Anti-Human Brachyury Monoclonal Antibody (Catalog # MAB20851) at a 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Brachyury is a 435 aa T-box family transcription factor. Human Brachyury shares 90% and 91% aa identity with mouse and rat Brachyury, respectively. It is required in the early determination and differentiation of mesoderm. Additionally, expression of Brachyury has been shown to be upregulated in a number of cancers, and in some cases, correlates with increased aggressiveness.
Citations for Human Brachyury Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
International Journal of Molecular Sciences, 2022;23(13):.
Sample Types: Whole Cells
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation
Authors: AM Drozd, L Mariani, X Guo, V Goitea, NA Menezes, E Ferretti
International Journal of Molecular Sciences, 2022;23(18):.
Sample Types: Whole Cells
7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome
Authors: H Tomita, KM Hines, JM Herron, A Li, DW Baggett, L Xu
Sample Types: Whole Tissue
Combined PDGFR and HDAC Inhibition Overcomes PTEN Disruption in Chordoma.
Authors: Lee D, Zhang Y, Kassam A, Park M, Gardner P, Prevedello D, Henry S, Horbinski C, Beumer J, Tawbi H, Williams B, Shaffrey M, Egorin M, Abounader R, Park D
PLoS ONE, 2015;10(8):e0134426.
Sample Types: Cell Lysates
Applications: Western Blot
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