Detection of Human BRDG1 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human BRDG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRDG1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BCR downstream-signaling protein 1 (BRDG1; also known as Signal-transducing adaptor protein 1, STAP1) is a 36‑37 kDa adaptor molecule that is involved in inflammation. It is expressed in hematopoietic cells, including B cells, macrophages and microglia. BRDG1 is believed to promote and prolong the immune response by controlling cell migration, secretion and phagocytosis. It does so by likely controlling the recruitment of signaling kinases to TRKs, and potentiating BCR-mediated activation of CREB and Elk‑1. Human BRDG1 is 295 amino acids (aa) in length and contains a plextrin homology domain that binds to membrane phospholipids (aa 25‑121), and one SH2 domain that recognizes phosphorylated Tyr residues (aa 176‑259). There is a Tec-mediated phosphorylation site at Tyr65. Over amino acids 177‑280, human BRDG1 shares 82% aa identity with mouse BRDG1.
BCR Downstream-signaling Protein 1
Entrez Gene IDs:
26228 (Human); 56792 (Mouse); 305269 (Rat)
BCR downstream-signaling protein 1; BRDG1STAP-1BCR downstream signaling 1; Docking protein BRDG1; signal transducing adaptor family member 1; signal-transducing adaptor protein 1; signal-transducing adaptor protein-1; STAP1; Stem cell adaptor protein 1
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