Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BRDG1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BRDG1
Ala177-Ser280
Accession # Q9ULZ2

Specificity

Detects endogenous human BRDG1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BRDG1 Antibody

Detection of Human BRDG1 antibody by Western Blot.

Detection of Human BRDG1 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human BRDG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRDG1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human BRDG1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BRDG1

BCR downstream-signaling protein 1 (BRDG1; also known as Signal-transducing adaptor protein 1, STAP1) is a 36‑37 kDa adaptor molecule that is involved in inflammation. It is expressed in hematopoietic cells, including B cells, macrophages and microglia. BRDG1 is believed to promote and prolong the immune response by controlling cell migration, secretion and phagocytosis. It does so by likely controlling the recruitment of signaling kinases to TRKs, and potentiating BCR-mediated activation of CREB and Elk‑1. Human BRDG1 is 295 amino acids (aa) in length and contains a plextrin homology domain that binds to membrane phospholipids (aa 25‑121), and one SH2 domain that recognizes phosphorylated Tyr residues (aa 176‑259). There is a Tec-mediated phosphorylation site at Tyr65. Over amino acids 177‑280, human BRDG1 shares 82% aa identity with mouse BRDG1.

Long Name

BCR Downstream-signaling Protein 1

Alternate Names

STAP1

Entrez Gene IDs

26228 (Human); 56792 (Mouse); 305269 (Rat)

Gene Symbol

STAP1

UniProt

Q9ULZ2

Additional BRDG1 Products

Product Documents for Human BRDG1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BRDG1 Antibody

For research use only

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